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Team:Brown/Notebook/July20
From 2010.igem.org
Tuesday, July 20, 2010
Results of blue-white selection for WillRS-pGEM ligation
We appear to have a good number of colonies (about 100-300), some white, some blue. No contamination.
- Put in the fridge (4C) for 2 hours and then pick a colony
Plan: put into 3ul amp into 3mL cultures and grow out; miniprep all; digest and run on gel to verify which ones worked.
Miniprep of overnight cultures
Bba_R0080 - pAraC
Bba_B0015 - Ter-Ter
Bba_I732100 - LacI
Notes
- Continue typing up notebook for wiki
- Prepare for poster presentation on Aug 5th
- Kan plates need to be redone once new Kan arrives
Made overnight culture of Lovtap r*2
MRS Broth-agar plates for miraculin expt.
- 27.5g of mrs broth and 5g of bacto agar in 500ml water
- Tet - 5ug/ml
- 250ul of 10mg/ml stock in 500ml liquid
