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Team:BCCS-Bristol/Wetlab/BioBricks
From 2010.igem.org
(New page: == Biobricks == '''Preliminary Experiments''' During the preliminary experiments we used the following biobricks [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] - pTet GFP This ...) |
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== Biobricks == | == Biobricks == | ||
| - | '''Preliminary Experiments''' | + | <u>'''Biobricks Used in Preliminary Experiments'''</u> |
| - | + | '''[http://partsregistry.org/Part:BBa_I13522 BBa_I13522]''' - pTet GFP | |
| - | + | GFP under the control of a constitutive promoter. This was used to visualise ''E.coli'' on soil under a fluorescence microscope during the preliminary testing stage. | |
| - | This | + | Vector used: pSB1A2. Ampicillin resistance used as selective marker. |
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| + | |||
| + | '''[http://partsregistry.org/Part:BBa_K216009 BBa_K216009]''' - PyeaR with lacZ' | ||
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| + | Nitrate- and nitrite-sensitive promoter upstream of lacZ' reporter. Used to characterize the promoter through a Miller assay. | ||
| + | |||
| + | Vector used: pSB1A2. Ampicillin resistance used as selective marker. | ||
| + | |||
| + | |||
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| + | <u>'''Biobricks to be used in the Project'''</u> | ||
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| + | '''[http://partsregistry.org/Part:BBa_K216005 BBa_K216005]''' - PyeaR promoter | ||
| + | |||
| + | Nitrate- and nitrite-sensitive promoter. This will be combined with [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] or [http://partsregistry.org/Part:BBa_E0240 BBa_E0240] to create a nitrate-sensitive system which signals through expression of GFP. | ||
| + | |||
| + | Vector used: pSB1A2. Ampicillin resistance used as selective marker. | ||
| + | |||
| + | |||
| + | '''[http://partsregistry.org/Part:BBa_E0840 BBa_E0840]''' - GFP generator | ||
| + | |||
| + | GFP plus strong RBS and termination sequences. | ||
| + | |||
| + | Vector used: pSB1A2. Ampicillin resistance used as selective marker. | ||
| + | |||
| + | |||
| + | '''[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]''' - GFP generator | ||
| + | |||
| + | GFP plus weaker RBS and termination sequences. | ||
| + | |||
| + | Vector used: pSB1A2. Ampicillin resistance used as selective marker. | ||
| + | |||
| + | |||
| + | '''[http://partsregistry.org/Part:BBa_J04450 BBa_J04450]''' - RFP coding device | ||
| + | |||
| + | RFP under the control of a constitutive promoter. This will be included in the ''E.coli'' chassis as a background fluorescence to aid in sensing and identifying the GFP signal, plus to act as a reference point for analysis of expression level of the GFP signal. | ||
| + | |||
| + | Vector used: pSB1K3. Kanamycin resistance used as selective marker. | ||
| + | |||
| + | |||
| + | '''[http://partsregistry.org/Part:BBa_I732017 BBa_I732017]''' - lacZ full-length (RBS+ TERM-) | ||
| + | |||
| + | Full length copy of lacZ which will be combined with PyeaR to further categorise the promoter used in our Nitrate sensor system. | ||
Latest revision as of 09:32, 12 August 2010
iGEM 2010
Biobricks
Biobricks Used in Preliminary Experiments
BBa_I13522 - pTet GFP
GFP under the control of a constitutive promoter. This was used to visualise E.coli on soil under a fluorescence microscope during the preliminary testing stage.
Vector used: pSB1A2. Ampicillin resistance used as selective marker.
BBa_K216009 - PyeaR with lacZ'
Nitrate- and nitrite-sensitive promoter upstream of lacZ' reporter. Used to characterize the promoter through a Miller assay.
Vector used: pSB1A2. Ampicillin resistance used as selective marker.
Biobricks to be used in the Project
BBa_K216005 - PyeaR promoter
Nitrate- and nitrite-sensitive promoter. This will be combined with BBa_E0840 or BBa_E0240 to create a nitrate-sensitive system which signals through expression of GFP.
Vector used: pSB1A2. Ampicillin resistance used as selective marker.
BBa_E0840 - GFP generator
GFP plus strong RBS and termination sequences.
Vector used: pSB1A2. Ampicillin resistance used as selective marker.
BBa_E0240 - GFP generator
GFP plus weaker RBS and termination sequences.
Vector used: pSB1A2. Ampicillin resistance used as selective marker.
BBa_J04450 - RFP coding device
RFP under the control of a constitutive promoter. This will be included in the E.coli chassis as a background fluorescence to aid in sensing and identifying the GFP signal, plus to act as a reference point for analysis of expression level of the GFP signal.
Vector used: pSB1K3. Kanamycin resistance used as selective marker.
BBa_I732017 - lacZ full-length (RBS+ TERM-)
Full length copy of lacZ which will be combined with PyeaR to further categorise the promoter used in our Nitrate sensor system.
