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Team:Alberta/Notebook/ReusablePlates
From 2010.igem.org
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We used 3 tyes of scintered plastic: | We used 3 tyes of scintered plastic: | ||
| - | :*3.0mm Ultra-Fine PE sheet | + | :*3.0mm Ultra-Fine PE sheet(14um pore-size) |
| - | :*6.0mm Fine-Grade PE Sheet | + | ::**ultra-fine grade should be a bacterial barrier |
| - | :*4.5mm Medium Grade PE Sheet | + | :*6.0mm Fine-Grade PE Sheet(30um pore-size) |
| + | ::**fine grade should also be a bacterial barrier but it was too hard to cut | ||
| + | :*4.5mm Medium Grade PE Sheet(88um pore-size) | ||
| + | |||
| + | Procedure: | ||
| + | |||
| + | #tested plastic's ability to absorb LB | ||
| + | |||
| + | :*June 22, 2010 added 10ml of LB and 40ul chloramphenicol to plates made from the 3.0mm and 4.5mm scintered plastics. Incubated in a 37C warm room overnight. | ||
| + | |||
| + | :*June 23, 2010 7ml LB left in 3.0mm ultra-fine and 8ml left in 4.5mm medium-grade | ||
| + | |||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Revision as of 06:51, 27 October 2010
Creating Inexpensive and Reusable Plates
(Abandoned)
The equipment and supplies required to sterilize and make plates are expensive and not usually available to a high school lab. We attempted to create plates which could easily be sterilized by a microwave or bleach.
Scintered Plastic Plates
Scintered Plastic Supplier: SPC technologies Ltd.
We used 3 tyes of scintered plastic:
- 3.0mm Ultra-Fine PE sheet(14um pore-size)
- ultra-fine grade should be a bacterial barrier
- 6.0mm Fine-Grade PE Sheet(30um pore-size)
- fine grade should also be a bacterial barrier but it was too hard to cut
- 4.5mm Medium Grade PE Sheet(88um pore-size)
Procedure:
- tested plastic's ability to absorb LB
- June 22, 2010 added 10ml of LB and 40ul chloramphenicol to plates made from the 3.0mm and 4.5mm scintered plastics. Incubated in a 37C warm room overnight.
- June 23, 2010 7ml LB left in 3.0mm ultra-fine and 8ml left in 4.5mm medium-grade
