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Team:Alberta/Kit components
From 2010.igem.org
(Difference between revisions)
| Line 24: | Line 24: | ||
! Violacein Pathway | ! Violacein Pathway | ||
! Transcription Factors | ! Transcription Factors | ||
| + | ! Resistance Marker | ||
|- | |- | ||
| Green Fluorescent Protein | | Green Fluorescent Protein | ||
| Line 29: | Line 30: | ||
| VioA | | VioA | ||
| LacI | | LacI | ||
| + | | Ampicillin | ||
|- | |- | ||
| Red Fluorescent Protein | | Red Fluorescent Protein | ||
| Line 34: | Line 36: | ||
| VioB | | VioB | ||
| AraC | | AraC | ||
| + | |Chloramphenicol | ||
|- | |- | ||
| Cyan Fluorescent Protein | | Cyan Fluorescent Protein | ||
| Line 39: | Line 42: | ||
| VioD | | VioD | ||
| LambdaCI | | LambdaCI | ||
| + | | Kanamycin | ||
|- | |- | ||
| Yellow Fluorescent Protein | | Yellow Fluorescent Protein | ||
| Line 44: | Line 48: | ||
| VioE | | VioE | ||
| TetR | | TetR | ||
| + | | Tetramycin | ||
|} | |} | ||
| Line 49: | Line 54: | ||
'''Linker Parts''' | '''Linker Parts''' | ||
| - | + | *Transcription | |
'''Other Parts''' | '''Other Parts''' | ||
Revision as of 02:16, 27 October 2010
The DNA Components
Open Reading Frame Parts
| Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
|---|---|---|---|---|
| Green Fluorescent Protein | CrtE | VioA | LacI | Ampicillin |
| Red Fluorescent Protein | CrtB | VioB | AraC | Chloramphenicol |
| Cyan Fluorescent Protein | CrtI | VioD | LambdaCI | Kanamycin |
| Yellow Fluorescent Protein | CrtY | VioE | TetR | Tetramycin |
Linker Parts
- Transcription
Other Parts
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- Lyophilized LB media
- Plates
- Iron Micro Beads
