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Team:Northwestern/protocol/

835 B (136 words) - 18:35, 16 October 2010
Team:Northwestern/Protocol/PDNA
Back to [[Team:Northwestern/Protocol|Protocol]] [[Category:Protocol]]

942 B (150 words) - 01:27, 23 October 2010
Protocol/7

0 B (0 words) - 17:15, 26 October 2010
Protocol/5

0 B (0 words) - 17:16, 26 October 2010
Protocol/6

0 B (0 words) - 17:16, 26 October 2010
Team:USTC/Project/protocol
== USTC2010 IGEM DNA Gel extraction protocol == == USTC2010 IGEM Transformation protocol ==

1 KB (141 words) - 21:36, 27 October 2010
Protocol/17

0 B (0 words) - 17:13, 26 October 2010
Protocol/1

0 B (0 words) - 17:17, 26 October 2010
Team:Northwestern/Protocol/Quikchange (from primers to colonies!
Back to [[Team:Northwestern/Protocol|Protocol]] [[Category:Protocol]]

2 KB (393 words) - 01:27, 23 October 2010
Team:Northwestern/Protocol/Kit to Stock Plasmid
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3 KB (449 words) - 01:25, 23 October 2010
Team:Northwestern/Protocol/Ethanol Precipitation
==Procedure: PREFERRED -20°C Protocol== ==Procedure: QUICKER -80°C Protocol==

4 KB (639 words) - 01:10, 23 October 2010
Team:Northwestern/Protocol/New Part Design(PCR)
The following protocol is for creating a new biobricks part from a protein coding sequence. Once you have obtained your primers, you can follow the usual PCR protocol using a High Fidelity polymerase. Digest and ligate as usual.

2 KB (327 words) - 01:11, 23 October 2010
Team:Northwestern/Protocol/Transformation
Back to [[Team:Northwestern/Protocol|Protocol]] [[Category:Protocol]]

1 KB (200 words) - 01:11, 23 October 2010
Team:Northwestern/Protocol/O/N Culture
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525 B (92 words) - 01:12, 23 October 2010
Team:Northwestern/Protocol/Preparation of Competent Cells

2 KB (298 words) - 01:16, 23 October 2010
Team:Northwestern/Protocol/LB Media
Back to [[Team:Northwestern/Protocol|Protocol]] [[Category:Protocol]]

218 B (31 words) - 01:19, 23 October 2010
Team:Northwestern/Protocol/Glycerol Stocks
*This protocol describes a method for storing bacterial cultures containing glycerol which Back to [[Team:Northwestern/Protocol|Protocol]]

2 KB (323 words) - 01:19, 23 October 2010
Team:Northwestern/Protocol/Restriction Enzyme Digests
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982 B (152 words) - 01:20, 23 October 2010
Team:Northwestern/Protocol/Plasmid Construction
...ning, substantial effort has been put into optimizing the reactions. This protocol describes the exact procedures and quality control techniques used to make Back to [[Team:Northwestern/Protocol|Protocol]]

4 KB (585 words) - 01:20, 23 October 2010
Team:Northwestern/Protocol/3A Assembly
... cell. This issue can be partially checked via the colony PCR step in the protocol though if the genomic DNA is of the same size as your assembly, then the si Back to [[Team:Northwestern/Protocol|Protocol]]

9 KB (1,418 words) - 01:21, 23 October 2010

Page text matches

Team:Heidelberg/Notebook/BSDesign/July
...B, without Amp); Preperation of competent cells according to the following protocol: * thawing of Huh-7, HeLa p4 and HEK-293 cells according to the following protocol

27 KB (3,832 words) - 03:17, 28 October 2010
2010.igem.org/Team:Harvard/vectors/notebook
<ul><li>Digested O1, O2, E3, E4, R1, R3 with HindIII and SpeI (Fast Digest Protocol - make link)</li> <li>Ran digests on 1% agarose gel (protocol link)</li>

20 KB (3,317 words) - 18:23, 22 July 2010
Team:Johns Hopkins/Notebook
==Protocol== ====Protocol====

32 KB (5,044 words) - 03:57, 28 October 2010
Team:Northwestern/Protocol
!align="center"|[[Team:Northwestern/Protocol|'''Protocol''']] [[Team:Northwestern/Protocol/PDNA|Prepping DNA from the kit plates]]

5 KB (500 words) - 01:23, 27 October 2010
Team:Alberta/Building Parts
=====Restriction Digest with NotI Protocol:===== =====Ligation Protocol:=====

30 KB (4,641 words) - 19:55, 19 August 2010
Team:Michigan/Oil Sands October
...- following [[Media:Static+biofilm+quantification.pdf|Alex's biofilm assay protocol]] ...ay on pH9 plate, following [[Media:Static+biofilm+quantification.pdf‎|my protocol]]:

15 KB (2,257 words) - 01:08, 27 October 2010
Team:Yale/Our Project/Notebook/Week 4
<li> Prior to designing thermocycler protocol, used oligocalc to get the following Tm values for the primers. </li> ... annealing temperature of 55˚C. Devised the following "phs" Thermocycler Protocol 

21 KB (3,342 words) - 02:50, 28 October 2010
Team:Michigan/Protocols
[[Media:OD600 Cell Dilution Protocol.pdf‎|OD600 Cell Dilution Protocol]] ...a:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]]

4 KB (498 words) - 00:01, 27 October 2010
Team:SDU-Denmark/labnotes9
''Protocol:'' [[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]] ''Protocol:'' [[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]] 

39 KB (5,907 words) - 10:23, 1 October 2010
Team:SDU-Denmark/labnotes10
'''Protocol:'''[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.3 CP1.3] '''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1][https://

27 KB (4,115 words) - 09:36, 30 September 2010
Team:SDU-Denmark/protocols
''Protocol'' ...mination of product size. Due to Taq's lack of proofreading, only use this protocol for determining sizes.

33 KB (5,192 words) - 03:58, 28 October 2010
Team:Peking/Notebook/Protocols
<html><div id="Protocol Title"> ...rotocols/SDS&WB">*SDS-PAGE & western blot protocol</a>         

12 KB (1,933 words) - 05:54, 26 October 2010
Team:NYMU-Taipei/Experiments/Protocols
Follow the protocol based on the kit used. ! Protocol !! GeneArt !! Qiagen !! Viogene

9 KB (1,489 words) - 14:23, 27 October 2010
Team:Michigan/Alex's Notebook
...ved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol Created protocol/plan for experiment. [[Media:Quorum+Sensing+Tables-1-.pdf|Testing AI-2 Res

20 KB (3,132 words) - 04:36, 4 October 2010
Team:KIT-Kyoto/Protocol/Japanese
.../Note|Notebook]] > [[Team:KIT-Kyoto/Protocol|Protocol]] > [[Team:KIT-Kyoto/Protocol/Japanese|Japanese]]</td></tr></table> == Protocol ==

10 KB (709 words) - 08:58, 9 October 2010
Team:Michigan/Oil Sands June July
*[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Protocol for starting overnight culture from -80°C freezer]] *[[Media:6-29-2010_Making_Frozen_Stoks.pdf|Protocol for making frozen stocks]]

13 KB (2,023 words) - 00:19, 27 October 2010
Team:Lethbridge/Notebook/Lab Work/June
...ollow [[Team:Lethbridge/Notebook/Protocols|competent cell transformation]] protocol to transform the following: ...b> Set up new ligations of pLacI and sRBS-Lum-dT according to Tom Knight's protocol. Previous ligation had very little DNA. 

76 KB (13,714 words) - 01:36, 25 October 2010
Team:Heidelberg/Notebook/miMeasure/July
*afterwards, RNA Extraction was performed according to the following protocol: '''Protocol'''

15 KB (2,038 words) - 00:54, 27 October 2010
Team:NYMU-Taipei/Experiments/Riboswitch
*digest again(because we used XP's protocol for SP) ! PCR Protocol !!

34 KB (4,745 words) - 23:56, 27 October 2010
Team:Michigan/Quorum Sensing
...ved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol *Followed protocol for [[Media:Protocol_Heat_Shock_Transformation_2.pdf|Transformation-heat sh

19 KB (2,960 words) - 01:14, 27 October 2010