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  • Team:Tokyo Tech/Project/Artificial Cooperation System/modeling
    ...al Cooperation System. Fourth, in the contrast, when the system worked and Kanamycin existed, the number of cell B increased after temporal decline (Fig.3-4-4). ...ch_kan_sim_result.jpg|300px|left|thumb|Fig3-4-4 when the system worked and Kanamycin existed (work done by Shohei Kitano) ]]
    9 KB (1,421 words) - 03:58, 28 October 2010
  • Team:Tsinghua/project/outline/m1
    In donor plasmid A, we insert only one gene, kanamycin resistant gene (Kanr) to test our recombination. At the 5’ end of Kanr, w In donor plasmid B, we insert two genes, kanamycin resistant gene (Kanr) and Chloromycetin resistant gene (Chlr). At the 5’
    20 KB (3,173 words) - 16:15, 27 October 2010
  • Team:Kyoto/Parts
    ...23-L||[http://partsregistry.org/Part:BBa_pSB1AK3 BBa_pSB1AK3]||Ampicillin, Kanamycin||129||3189 ...Ba_J23101]||2-17-F||[http://partsregistry.org/Part:BBa_pSB3K3 BBa_pSB3K3]||Kanamycin||919||2750
    9 KB (1,303 words) - 22:47, 27 October 2010
  • Team:Michigan/Quorum Sensing
    7/17 plates are no good -- they needed kanamycin *broth cultures had kanamycin added a day later -- too late to be cryostored, but they were transfered to
    19 KB (2,960 words) - 01:14, 27 October 2010
  • Team:Kyoto/Protocols
    #* Kanamycin: #*; Kanamycin: 0.5g
    19 KB (3,027 words) - 02:42, 28 October 2010
  • Team:Alberta/Notebook July
    ...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w ...We picked as many colonies as possible and streaked on both LB plates with Kanamycin and Chloramphenicol resistance and LB plates with only Choramphenicol resis
    30 KB (4,765 words) - 22:55, 10 August 2010
  • Team:Alberta/Notebook August
    ...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w ...We picked as many colonies as possible and streaked on both LB plates with Kanamycin and Chloramphenicol resistance and LB plates with only Choramphenicol resis
    26 KB (3,927 words) - 21:02, 10 August 2010
  • Team:Michigan/Alex's Notebook
    7/17 plates are no good -- they needed kanamycin *broth cultures had kanamycin added a day later -- too late to be cryostored, but they were transfered to
    20 KB (3,132 words) - 04:36, 4 October 2010
  • Team:Michigan/Pili August September
    2. Added 5 microliters of Kanamycin to each of the 50 mL tubes in step 1. *Marc adds 50uL of kanamycin onto each LB-agar plate and spread evenly with sterile glass beads
    12 KB (1,774 words) - 03:05, 27 October 2010
  • Team:NCTU Formosa/Temperature Control
    ...n the appropriate media and antibiotic, such as agar plates with 25 µg/ml kanamycin.<br> ...2% casamino acids, 1 mM MgSO4, 2 μM thiamine, 10 mM glucose, and 50 ng/ml Kanamycin antibiotic.)<br>
    22 KB (2,885 words) - 02:18, 28 October 2010
  • Team:Calgary/3 August 2010
    ...ot grow of both regular Top10 cells and ccdB resistant cells on ampicillin-kanamycin and ampicillin-chloramphenicol plates.
    3 KB (587 words) - 03:01, 27 October 2010
  • Team:Waterloo
    ...tor for both the measured part and the standard is pSB3K3 (low copy number kanamycin resistant BioBrick plasmid) with green fluorescent protein gene as reporte I20260 on pSB3K3 inocluated into Kanamycin LB<br />
    152 KB (22,732 words) - 17:38, 23 November 2010
  • Team:Caltech/Week 2
    ...e kanamycin test grew bacteria, suggesting that there is an issue with the kanamycin stock, which we will have to remake. ** Set up antibiotic test plates for kanamycin (kan) & tetracycline (tet), just as on [https://2010.igem.org/Team:Caltech/
    8 KB (1,258 words) - 19:00, 21 July 2010
  • Team:TU Delft/6 July 2010 content
    * rubB (Kanamycin) * ALDH (Kanamycin)
    5 KB (699 words) - 19:23, 2 August 2010
  • Team:BCCS-Bristol/Wetlab/Notebook
    ...://partsregistry.org/Part:BBa_E0240 BBa_E0240] transformations, and agar + Kanamycin for [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] transformation. L ...g the [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] biobrick are ''Kanamycin'' resistant, not Ampicillin resistant. Will reattempt overnight culture on
    17 KB (2,636 words) - 19:06, 16 August 2010
  • Team:Alberta/Notebook June
    ...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w <ul>14&mu;L either A/B' or B/A' Kanamycin resistance cassette (approx. 100ng/&mu;L)</ul>
    37 KB (5,929 words) - 18:34, 4 August 2010
  • Team:Harvard/vectors/vectors
    ...nfers plant resistance to <a href="http://en.wikipedia.org/wiki/Kanamycin">kanamycin</a>, to be used in transformant selection.</p></td> ...r, and pORE R3 the smgfp reporter. Both vectors confer plant resistance to kanamycin. </p></td>
    4 KB (596 words) - 21:37, 24 October 2010
  • Team:Bielefeld-Germany/Project/Approach
    ..., a ''vir'' promoter and a readout or selection gene (luciferase, mRFP and kanamycin resistance, respectively): ...stems for the detection of the chosen substances. By varying the amount of kanamycin we will be able to carefully select some promising clones out of thousands
    8 KB (1,195 words) - 03:10, 28 October 2010
  • Team:Calgary/Notebook/Safety And Protocols
    ...o;L of SOC (30 minutes for Ampicilin resistant plasmids and 60 minutes for kanamycin resistant plasmids) </li> <li> Add Antibiotic (10 &micro;L Ampicillin, 5 &micro;L Kanamycin, or 3 &micro;L Chloramphenicol)</li>
    27 KB (4,452 words) - 10:30, 27 October 2010
  • Team:Freiburg Bioware/testpage
    * Kanamycin: 1 g Kanamycin was dissolved in 20 mL multipore-H2O, sterilized by filtration and filled i Further on two control plates containing ampicillin or kanamycin were prepared. Non-transformed cells were plated on them and also stored ov
    20 KB (2,960 words) - 12:06, 6 September 2010

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