User contributions
From 2010.igem.org
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- 08:13, 24 October 2010 (diff | hist) N Minnesota-experimental/15 September 2010 (New page: '''Annie''' Inoculated overnight cultures of psB3K pLac into LB. Transfered two of the inoculations to two new LB tubes.) (top)
- 02:04, 24 October 2010 (diff | hist) Minnesota-experimental/14 September 2010 (top)
- 02:04, 24 October 2010 (diff | hist) N Minnesota-experimental/14 September 2010 (New page: '''Tony''' Ran S, M, N, L, K, SMNLK, and GFP out on gel. Miniprep plux with elution in fifty microliters EB. Digestion of plux with EcoRI and SpeI.)
- 02:02, 24 October 2010 (diff | hist) N Minnesota-experimental/13 September 2010 (New page: '''Matt''' Transformations of BBa J23102 and BBa J23116. '''Tony''' Inoculated plux cultures overnight.) (top)
- 02:01, 24 October 2010 (diff | hist) N Minnesota-experimental/8 September 2010 (New page: '''Tony''' Transformations of pucBB EutS, M, N, L, K and EutSMNLK and pucBB GFP.) (top)
- 02:01, 24 October 2010 (diff | hist) N Minnesota-experimental/7 September 2010 (New page: '''Ian Made new competent cells. CAT ligase free cloning with pucBB CAT BglII/NotI. Also made fifteen ampicillin plates.) (top)
- 01:57, 24 October 2010 (diff | hist) N Minnesota-experimental/2 September 2010 (New page: '''Ian''' PCR of CAT product and PCR of pichia genomic DNA.) (top)
- 01:56, 24 October 2010 (diff | hist) N Minnesota-experimental/31 August 2010 (New page: '''Tony''' PCR reactions of AOX and CAT from Pichia genomic DNA.) (top)
- 01:55, 24 October 2010 (diff | hist) N Minnesota-experimental/30 August 2010 (New page: '''Ian''' Tested competent cells w/pucBB GFP. Ran PCR and gel of AOX and CAT.) (top)
- 01:36, 24 October 2010 (diff | hist) N Minnesota-experimental/25 August 2010 (New page: '''Ian''' Ran digest of puc BB AOX with BglII/NotI. Gel confirms that fragment is not the correct size) (top)
- 01:32, 24 October 2010 (diff | hist) N Minnesota-experimental/24 August 2010 (New page: '''Ian''' PCR reaction in order to check presence of AOX gene.) (top)
- 01:31, 24 October 2010 (diff | hist) N Minnesota-experimental/23 August 2010 (New page: '''Ian''' Ligation of Eut SK and psB1C3 (twenty microliter reaction).) (top)
- 01:30, 24 October 2010 (diff | hist) N Minnesota-experimental/21 August 2010 (New page: '''Ian''' PCR purification of digest miniprep of AOX ligation.) (top)
- 01:30, 24 October 2010 (diff | hist) N Minnesota-experimental/20 August 2010 (New page: '''Matt''' Overnight cultures of pucBB-AOX colonies. '''Ian''' Ran digest of Eut SK PCR with EcoRI/PstI and ran out on successful gel.) (top)
- 01:27, 24 October 2010 (diff | hist) N Minnesota-experimental/18 August 2010 (New page: '''Ian''' Ran digest of psB3K3 Eut SK with PstI/EcoRI. Ran successful PCR and gel of digest.) (top)
- 01:26, 24 October 2010 (diff | hist) N Minnesota-experimental/17 August 2010 (New page: '''Matt''' Ligation of AOX and CAT genes into pucBB.) (top)
- 01:25, 24 October 2010 (diff | hist) N Minnesota-experimental/16 August 2010 (New page: '''Matt''' Transformation of Pichia AOX and CAT. No reaction observed. Digestion of AOX and CAT PCR DNA with BglII/NotI and placed in thirty seven degree overnight. '''Tony''' PCR for DA...) (top)
- 01:23, 24 October 2010 (diff | hist) N Minnesota-experimental/6 August 2010 (New page: '''Tony''' Spun down colonies and stored two of them in freezers. No visible gel result from colony PCR reaction.) (top)
- 01:22, 24 October 2010 (diff | hist) N Minnesota-experimental/5 August 2010 (New page: '''Tony and Matt''' Ran gel of AOX and CAT genes. Reaction successful. Also ran colony PCR in order to confirm appearance of pucBB-DAS colonies. '''Ian''' PCR reaction of psB3K3 pLac-GFP...) (top)
- 01:14, 24 October 2010 (diff | hist) N Minnesota-experimental/4 August 2010 (New page: '''Tony''' Nanodrop concentrations of pucBB NotI/BglII and DAS PCR product. Ligase free cloning of DAS pCR product and pucBB BglII/NotI. Second attempt at PCR in order to isolate AOX and...) (top)
- 01:10, 24 October 2010 (diff | hist) N Minnesota-experimental/3 August 2010 (New page: '''Tony and Matt''' Ran gel of PCR resutls. Successfully amplified the DAS genes but still failed to isolated CAT genes and AOX genes. '''Ian''' Minipreps of psB3K3 pLac GFP.) (top)
- 01:01, 24 October 2010 (diff | hist) N Minnesota-experimental/2 August 2010 (New page: '''Matt and Tony''' PCR reaction of Pichia genes AOX, CAT and DAS. Used positive control EutG GFP.) (top)
- 00:58, 24 October 2010 (diff | hist) N Minnesota-experimental/31 July 2010 (New page: '''Ian''' Transformation of pLac and GFP/psB3K3 ligation. Sent psB3K3 eut SK, BglII Eut MN-F for sequencing to University of Minnesota BioMedical Genomics Center.) (top)
- 00:56, 24 October 2010 (diff | hist) N Minnesota-experimental/29 July 2010 (New page: '''Ian''' Ran digest of pLac with EcoRI/SpeI.) (top)
- 00:55, 24 October 2010 (diff | hist) N Minnesota-experimental/28 July 2010 (New page: '''Annie''' Ran gel of previous day PCR. Inoculated Pichia from Pichia plate in YPD and kept in thirty degree shaker. '''Ian''' Ran gel of GFP fragment (XbaI/PstI). Transformation of psB...) (top)
- 00:52, 24 October 2010 (diff | hist) N Minnesota-experimental/27 July 2010 (New page: '''Annie''' Test gel run of PCR products. PCR of genomic DNA (reaction failed). '''Tony''' Third attempt at PCR of Pichia genes (AOX, CAT, and DAS). '''Ian''' Miniprep of overnight cultur...) (top)
- 00:49, 24 October 2010 (diff | hist) N Minnesota-experimental/26 July 2010 (New page: '''Tony and Matt''' PCR reactions of AOX, DAS, and CAT.) (top)
- 00:48, 24 October 2010 (diff | hist) Minnesota-experimental/22 July 2010 (top)
- 00:39, 24 October 2010 (diff | hist) Minnesota-experimental/22 July 2010
- 00:39, 24 October 2010 (diff | hist) N Minnesota-experimental/22 July 2010 (New page: '''Annie''' Genomic extraction of pichia. '''Mat''' Transformation of pacBB EutG DsRed ligation in thirty seven degree incubator.)
- 00:38, 24 October 2010 (diff | hist) N Minnesota-experimental/21 July 2010 (New page: '''Annie''' Inoculated four microliters Pichia in fifty microliters YPD media. Plated on YPD media for future inoculation. '''Tony and Matt''' Colony PCR screen to find pacBB EutG DsRed c...) (top)
- 00:34, 24 October 2010 (diff | hist) Minnesota-experimental/20 July 2010 (top)
- 00:32, 24 October 2010 (diff | hist) N Minnesota-experimental/19 July 2010 (New page: '''Tony''' Ligation of pacBB EutG DsRed. Cultivated two colonies of pacBB EutC DsRed in LB and left overnight at thirty seven degrees Celsius. '''Ian''' Transformation of psB3K3 Eut LKMNS...) (top)
- 00:29, 24 October 2010 (diff | hist) N Minnesota-experimental/16 July 2010 (New page: '''Ian''' Ligation of Eut LKMNS with psb3K5 (twenty microliters).) (top)
- 00:28, 24 October 2010 (diff | hist) N Minnesota-experimental/20 July 2010 (New page: '''Annie''' Made YDP media and inoculated PIchia Pastoris. '''Tony''' PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eut...)
- 00:26, 24 October 2010 (diff | hist) N Minnesota-experimental/15 July 2010 (New page: '''Annie''' Minipreps of pacBBGGFP. Two samples sent for sequencing at University of Minnesota BioMedical Genomics Center. '''Ian''' PCR purification of LKMNS insert.) (top)
- 00:22, 24 October 2010 (diff | hist) N Minnesota-experimental/14 July 2010 (New page: '''Ian''' Minipreps set up of overnight cultures. Ligation reaction set up of pacBB-EutC-DsRed. Digestion reaction set up of pucBB-Eut LKMNS.) (top)
- 00:21, 24 October 2010 (diff | hist) Minnesota-experimental/13 July 2010 (top)
- 00:20, 24 October 2010 (diff | hist) N Minnesota-experimental/13 July 2010 (New page: '''Annie''' Transformations of pacBB and GGFP ligation reaction on CM plates. Digestion of pucBB GGFP with BglII/NotI overnight at thirty seven degree incubator. '''Matt''' Minipreps of T...)
- 00:16, 24 October 2010 (diff | hist) N Minnesota-experimental/12 July 2010 (New page: '''Annie''' Set up pacBB GGFP ligation reaction (BglII/NotI). '''Matt''' Overnight cultures of pacBB-EutG-dsRed overnight at thirty seven degrees Celsius incubator. '''Tony''' Ligation of ...) (top)
- 00:13, 24 October 2010 (diff | hist) N Minnesota-experimental/9 July 2010 (New page: '''Annie''' Ran gel pucBB GGFP digest and GGFP. '''Tony''' Purified digested Eut C DsRed BglII/NotI fragment. Eluted in thirty microliter buffer EB. '''Ian''' Made nine CM plates. Transf...) (top)
- 00:11, 24 October 2010 (diff | hist) N Minnesota-experimental/8 July 2010 (New page: '''Annie''' Minipreps of pucBB GGFP. Nanodrop concentrations of pucBB GGFP. Digestion of pucBB GGFP with BglII/NotI. '''Tony and Ian''' Nanodrop concentration of pacBB EutG DsRed. Ligat...) (top)
- 00:08, 24 October 2010 (diff | hist) Minnesota-experimental/7 July 2010 (top)
- 00:04, 24 October 2010 (diff | hist) N Minnesota-experimental/7 July 2010 (New page: '''Annie''' PCR reactions set up for EutC and EutG. Overnight cultures set up for pucBB GGFP in thirty seven degree incubator.)
- 00:01, 24 October 2010 (diff | hist) N Minnesota-experimental/6 July 2010 (New page: '''Annie''' Set up transformations for all three ligations (pac EutC DsRed, pacBB EutG DsRed, pacBB EutC GFP).) (top)
- 00:00, 24 October 2010 (diff | hist) N Minnesota-experimental/1 July 2010 (New page: '''Annie''' PCR purification of Eut G DsREd, Eut C DsRed, and Eut G GFP. '''Tony''' Minipreps of pacBB plasmid. Eluted in forty microliters DH2O (four tubes). '''Ian''' Nanodrop concentra...) (top)
- 23:57, 23 October 2010 (diff | hist) N Minnesota-experimental/30 June 2010 (New page: '''Annie''' PCR purification of Eut C DsRed and Eut G DsRed from previous day digestion. PCR reaction of of EutC dsRed. Digestion reaction of Eut G GFP with BglII/NotI. '''Ian''' Ran gel...) (top)
- 23:45, 23 October 2010 (diff | hist) N Minnesota-experimental/29 June 2010 (New page: '''Annie''' Minipreps of pac and GFP. Overnight digest of DsREd Eut C and Eut G with BglII/NotI. '''Matt''' Digestion of pacBB-GFP with BglII/NotI (fifty microliter reaction). Placed in ...) (top)
- 23:42, 23 October 2010 (diff | hist) Minnesota-experimental/28 June 2010 (top)
- 23:41, 23 October 2010 (diff | hist) Minnesota-experimental/28 June 2010
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