User contributions
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- 08:13, 24 October 2010 (diff | hist) N Minnesota-experimental/15 September 2010 (New page: '''Annie''' Inoculated overnight cultures of psB3K pLac into LB. Transfered two of the inoculations to two new LB tubes.) (top)
- 02:04, 24 October 2010 (diff | hist) Minnesota-experimental/14 September 2010 (top)
- 02:04, 24 October 2010 (diff | hist) N Minnesota-experimental/14 September 2010 (New page: '''Tony''' Ran S, M, N, L, K, SMNLK, and GFP out on gel. Miniprep plux with elution in fifty microliters EB. Digestion of plux with EcoRI and SpeI.)
- 02:02, 24 October 2010 (diff | hist) N Minnesota-experimental/13 September 2010 (New page: '''Matt''' Transformations of BBa J23102 and BBa J23116. '''Tony''' Inoculated plux cultures overnight.) (top)
- 02:01, 24 October 2010 (diff | hist) N Minnesota-experimental/8 September 2010 (New page: '''Tony''' Transformations of pucBB EutS, M, N, L, K and EutSMNLK and pucBB GFP.) (top)
- 02:01, 24 October 2010 (diff | hist) N Minnesota-experimental/7 September 2010 (New page: '''Ian Made new competent cells. CAT ligase free cloning with pucBB CAT BglII/NotI. Also made fifteen ampicillin plates.) (top)
- 01:57, 24 October 2010 (diff | hist) N Minnesota-experimental/2 September 2010 (New page: '''Ian''' PCR of CAT product and PCR of pichia genomic DNA.) (top)
- 01:56, 24 October 2010 (diff | hist) N Minnesota-experimental/31 August 2010 (New page: '''Tony''' PCR reactions of AOX and CAT from Pichia genomic DNA.) (top)
- 01:55, 24 October 2010 (diff | hist) N Minnesota-experimental/30 August 2010 (New page: '''Ian''' Tested competent cells w/pucBB GFP. Ran PCR and gel of AOX and CAT.) (top)
- 01:36, 24 October 2010 (diff | hist) N Minnesota-experimental/25 August 2010 (New page: '''Ian''' Ran digest of puc BB AOX with BglII/NotI. Gel confirms that fragment is not the correct size) (top)
- 01:32, 24 October 2010 (diff | hist) N Minnesota-experimental/24 August 2010 (New page: '''Ian''' PCR reaction in order to check presence of AOX gene.) (top)
- 01:31, 24 October 2010 (diff | hist) N Minnesota-experimental/23 August 2010 (New page: '''Ian''' Ligation of Eut SK and psB1C3 (twenty microliter reaction).) (top)
- 01:30, 24 October 2010 (diff | hist) N Minnesota-experimental/21 August 2010 (New page: '''Ian''' PCR purification of digest miniprep of AOX ligation.) (top)
- 01:30, 24 October 2010 (diff | hist) N Minnesota-experimental/20 August 2010 (New page: '''Matt''' Overnight cultures of pucBB-AOX colonies. '''Ian''' Ran digest of Eut SK PCR with EcoRI/PstI and ran out on successful gel.) (top)
- 01:27, 24 October 2010 (diff | hist) N Minnesota-experimental/18 August 2010 (New page: '''Ian''' Ran digest of psB3K3 Eut SK with PstI/EcoRI. Ran successful PCR and gel of digest.) (top)
- 01:26, 24 October 2010 (diff | hist) N Minnesota-experimental/17 August 2010 (New page: '''Matt''' Ligation of AOX and CAT genes into pucBB.) (top)
- 01:25, 24 October 2010 (diff | hist) N Minnesota-experimental/16 August 2010 (New page: '''Matt''' Transformation of Pichia AOX and CAT. No reaction observed. Digestion of AOX and CAT PCR DNA with BglII/NotI and placed in thirty seven degree overnight. '''Tony''' PCR for DA...) (top)
- 01:23, 24 October 2010 (diff | hist) N Minnesota-experimental/6 August 2010 (New page: '''Tony''' Spun down colonies and stored two of them in freezers. No visible gel result from colony PCR reaction.) (top)
- 01:22, 24 October 2010 (diff | hist) N Minnesota-experimental/5 August 2010 (New page: '''Tony and Matt''' Ran gel of AOX and CAT genes. Reaction successful. Also ran colony PCR in order to confirm appearance of pucBB-DAS colonies. '''Ian''' PCR reaction of psB3K3 pLac-GFP...) (top)
- 01:14, 24 October 2010 (diff | hist) N Minnesota-experimental/4 August 2010 (New page: '''Tony''' Nanodrop concentrations of pucBB NotI/BglII and DAS PCR product. Ligase free cloning of DAS pCR product and pucBB BglII/NotI. Second attempt at PCR in order to isolate AOX and...) (top)
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