INSA-Lyon/30 September 2010
From 2010.igem.org
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- | <p> | + | <p>(mise en collection) of I0500, 10L and 4O </p><br> |
+ | <p>Agarose gel electrophoris : <br> | ||
+ | <ol id="list_extra"> | ||
+ | <li>8C/14K :ok (mise en collection)</li> | ||
+ | <li>8C : nothing (!?)</li> | ||
+ | <li>pha B : OK, purification of the band</li> | ||
+ | </ol><br></p> | ||
+ | <p>Digestion : <br> | ||
+ | <ol id="list_extra"> | ||
+ | <li>18A, phaA : with E+S</li> | ||
+ | <li>14/12/13, pha B and L2/1N : with X+P</li> | ||
+ | </ol><br></p> | ||
+ | <p>Agarose gel electrophoris for purification : we can't see anything so we will do it again later</p><br> | ||
+ | <p>DNA extraction, digestion with E and agarose gel electrophoris for the 10 clones of 1F/24A/14K : the higher weigh were not separated, to do again</p> | ||
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Revision as of 11:26, 8 October 2010
September 30th
(mise en collection) of I0500, 10L and 4O
Agarose gel electrophoris :
- 8C/14K :ok (mise en collection)
- 8C : nothing (!?)
- pha B : OK, purification of the band
Digestion :
- 18A, phaA : with E+S
- 14/12/13, pha B and L2/1N : with X+P
Agarose gel electrophoris for purification : we can't see anything so we will do it again later
DNA extraction, digestion with E and agarose gel electrophoris for the 10 clones of 1F/24A/14K : the higher weigh were not separated, to do again
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