INSA-Lyon/29 July 2010
From 2010.igem.org
July 29
Digestion of 1N by NheI in order to compare with the digestion of L1 by NheI
Result of the L3 transformation : 22 big colonies and 37 small ones
Preculture of 10 colonies of L3 and 18A
Extraction, digestion with NotI and verification with an agarose gel electrophoris of the 10 clones from the ligation 1F/24A :
only the number 9 and 10 show a thin band at the right weight. New digestion of ninth and tenth clone with E+P
Digestion of 1F with S+P and ligation with 24A overnight
Digestion of 14K with X+P and purification with an electrophoris gel
Purification of 10 new clones of the ligation 1F/24A from the 26th july
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