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INSA-Lyon/27 September 2010
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Latest revision as of 20:05, 25 October 2010
September 27th
Digestion of the clones of 8C/14K (2 purifications). Agarose gel electrophoresis for weight verification : the ligation is OK
Liquid culture of 1F/24A, 14K and 1F/24A/14K
6 liquid cultures of PHL1273 : shacking speed 100 rpm and 3 in a waterbath at 30°C, 3 at 31°C
Gel purification for Tet and Cm plasmids: 30µl are deposited in two lanes per PCR product. 60mg agarose, for both (in 120µl NT), were extracted with NucleoSpin ExtractII kit (MN). Elution in 50µl, stored at -20°C.
Liquid cultures of Curli/22B in LB-Tet; L2/1N in LB-Cm; 18A in LB-Amp: 37°C overnight.
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