INSA-Lyon/23 July 2010
From 2010.igem.org
July 23
DNA Extraction 18A1, 1N1, 14K1, 23L1, 1F, 24A (concentrated in 40uL)
Agarose gel electrophoresis --> all extractions are OK
Digestions :
- 18A : S+E (30uL)
- 1N: X+P (34uL)
- 14K: E+S (30uL)
- 23L: E+X (30uL)
- 1F: S+P (40uL)
- 24A: X+P (40uL)
Agarose gel electrophoresis : no DNA for 23L
Second digestion of 23L (5uL) with 0,5uL of EcoR1 and 1uL of Xba1 -> gel OK
Ligations :
- 14K/23L
- ratio 2:1 --> 10/5µL
- ratio 4:1 --> 12/3µL
- 18A/1N: method 3A (10µL of each and one tube with 2,5µL or 5µL of plasmid C)
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