INSA-Lyon/22 September 2010
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- | <p>Ligation overnight of 8C/14K and 1F/24A/14K</p><br> | + | <p>Ligation overnight of : <br> |
+ | <ol id="list_extra"> | ||
+ | <li>8C/14K : one with only 14K from gel purification and one with the 8C and 14K from a gel purification</li> | ||
+ | <li>1F/24A/14K : one ligation</li> | ||
+ | </ol></p><br> | ||
<p>Liquid cultur of 18A/24A, 1F/24A, 14K, I0500 and PHL818</p><br> | <p>Liquid cultur of 18A/24A, 1F/24A, 14K, I0500 and PHL818</p><br> | ||
<p>Measure of fluorescence and DO of the liquid cultur of PHL1273 of the 20/09. Start of 6 new culturs : <br> | <p>Measure of fluorescence and DO of the liquid cultur of PHL1273 of the 20/09. Start of 6 new culturs : <br> |
Revision as of 15:41, 5 October 2010
September 22nd
DNA extraction of the clone of the ligation from the 21/09 of phaA/phaB
Agarose gel electrophoris for weight verification :
- 1F/24A : the clone (mise en )collection is OK
- Curli/22B : the clone (mise en )collection is OK
- 8C/14K : problem with the extraction (no trace of DNA on the gel)
Digestion and purification on agarose gel:
- 8C, 1F/24A : with S+P
- 14K with X+P
Ligation overnight of :
- 8C/14K : one with only 14K from gel purification and one with the 8C and 14K from a gel purification
- 1F/24A/14K : one ligation
Liquid cultur of 18A/24A, 1F/24A, 14K, I0500 and PHL818
Measure of fluorescence and DO of the liquid cultur of PHL1273 of the 20/09. Start of 6 new culturs :
- 3 in a water bath at 35°C, shaking speed 100 rpm
- 3 in a water bath at 36°C, shaking speed 100 rpm
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