INSA-Lyon/14 September 2010
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Revision as of 08:26, 17 September 2010
September 14th
Ligation :
- 18A+L3 in Cl backbone
- 14+12/13 : in Cl backbone
- Curli+22B in Tet backbone
Digestion :
- pha A/pha B (clones 7 to 10) with E
- 8C, I0500 : with E+S
- 14K, 22B : with X+P
Transformation of the plasmid with phasin-intein
Agarose gel electrophoris for verification :
- Clones 7 to 10 of the pha A/pha B ligation : none contain the ligation
- Digestion of 1F : ok
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