INSA-Lyon/14 October 2010

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Revision as of 20:17, 25 October 2010





October 14th

Transformation of the ligation of the 12/10 (with purified DNA)


Liquid culture of the ligation of the 12/10 (with unpurified DNA) :

  1. phasin/phasin-intein : 4 clones
  2. phasin-intein/2P: 7 clones
  3. phaC/12/13 and Curli/phaC: too much clones, the ligation doesn't success
  4. 18A/L2/1N : no clones

Digestion of the clones with X+P








June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

Retrieved from "http://2010.igem.org/INSA-Lyon/14_October_2010"