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HKU-Hong Kong/30 July 2010
From 2010.igem.org
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=='''Work schedule 30 July 2010'''== | =='''Work schedule 30 July 2010'''== | ||
*confirmation of selected C1 C2 as transformed cell | *confirmation of selected C1 C2 as transformed cell | ||
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===Enzyme digestion=== | ===Enzyme digestion=== | ||
Two bands were expected. Only one band of large size can be observed for both colonies, this is consistent with the conclusion drawn from the colony PCR. | Two bands were expected. Only one band of large size can be observed for both colonies, this is consistent with the conclusion drawn from the colony PCR. | ||
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Revision as of 18:28, 27 October 2010
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Contents |
Work schedule 30 July 2010
- confirmation of selected C1 C2 as transformed cell
- colony PCR/enzyme digestion of the 2 colonies (NcoI cut)
Results
Colony PCR
No bands can be observed. The two colonies are probably contaminations.
Enzyme digestion
Two bands were expected. Only one band of large size can be observed for both colonies, this is consistent with the conclusion drawn from the colony PCR.
By the HKU-Hong Kong iGEM team 2010
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