Team:NYU/Programming

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Biobricking Primers

This script allows the user to input a DNA sequence and outputs the primers necessary for biobricking that sequence via PCR. The script calculates Tm but does not (yet) consider primer dimers.

Finally got the CGI working - go [http://russelldurrett.com/biobrickprimers.html here for an online version! ]

[http://openwetware.org/images/a/a7/Biobrickprimers.txt Biobricking Primers Source]

Overlap Oligo Maker

Some very fast and efficient DNA assembly methods require parts to overlap in their sequences. This script will take two DNA sequences and output the oligos (and their Tms) one would need to PCR constructs with overlapping ends.

I'm a CGI machine! [http://www.russelldurrett.com/overlapoligos.html Check out the new and improved interactive overlap oligo maker.]

[http://openwetware.org/images/6/67/Overlapoligos.txt Overlap Oligos Source]

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