Team:HokkaidoU Japan/Notebook/September10

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Notebook

3 piece ligation, continued

  • Yesterday after purification by gel extraction electrophoresis showed very weak bands
  • This time increased PCR cycles to 40
  • Everything else was same as last time
  • But again it was a failure
  • Thought that template went bad，so used PCR Product as template
  • Extension was 90 sec, did 30 cycles
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