Team:TU Delft/11 June 2010 content

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Contents

Lab work

BioBrick stocks

Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.

The following plasmid concentrations were obtained:

BioBrick Concentration (ng/μL)
pSB3T5 266.0
pSB1T3 414.0
pSB1C3 284.7
pSB3C5 329.3
pSB1K3 270.1
pSB1A3 271.9
I13401 232.1
Ramon & Hugo in the lab
Thias doing labwork







Characterization of Anderson RBS sequences

Next to that we also started digesting the first parts we will be characterizing.

Strain Characterization

By Hugo

I prepared the cell bank according to the following procedure:

  • Spin 5 ml of the overnight culture
  • Add 500 ul of LB medium
  • Add 500 ul of 80% glycerol
  • Store the tube at -80ºC

Now, we have a cell bank ready to use!!!

Spring Workshop

Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.

Leaving Delft
Dinner on the way to Évry, while watching the soccer match in Gent
pizza tast good
Dinner on the way to Évry
Last car arriving at the hotel in Évry