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Team:HokkaidoU Japan/Notebook/September9
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{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September8|September 8]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September10|September 10]]</div></div> | {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September8|September 8]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September10|September 10]]</div></div> | ||
| - | =HSP, GFP | + | =3 piece ligation of HSP, GFP and pSB1C3= |
| + | |||
[[Image:HokkaidoU Japan 20100909a.jpg|200px|right|thumb|]] | [[Image:HokkaidoU Japan 20100909a.jpg|200px|right|thumb|]] | ||
{|style="text-align: center;" | {|style="text-align: center;" | ||
Revision as of 18:01, 27 September 2010
since 13 Jul 2010
since 1 Aug 2010
3 piece ligation of HSP, GFP and pSB1C3
| HSP | PCRしたもの | ゲル抽 | Digestion |
| GFP | 前日にゲル抽キットで精製した | － | Digestion |
| pSB1C3 | PCR後，ゲル抽精製したもの | － | Digestion |
- HSPのゲル抽のついでにGFPを電気泳動したところ，薄かったためこれもゲル抽することにした
- HSPとGFPのゲル抽後，電気泳動したところ，どちらも今後の操作には薄すぎたため，後日PCRからやりなおすことにした
