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Team:Wisconsin-Madison/encryption
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==Description== | ==Description== | ||
===Abstract=== | ===Abstract=== | ||
| + | Sequential logic switches are the basis for many common electronic devices such as digital clocks and calculators. Here we present a novel design for the imitation of sequential logic using basic genetic parts within E. Coli. By using a combination of DNA recombinase enzymes, promoter systems, and an innovative pattern of recombinase binding sites, we can reproduce sequential-logical functions on the compact molecular scale. By using single DNA molecules as a medium for such functions within bacterial vehicles, we can essentially mimic the functionality of a combination lock, and produce a "locked" gene which can be effectively "unlocked" only after a specific sequence of inputs detected by the bacterial promoter system. Since the DNA molecule is used as a logical medium, the "locked" and "unlocked" states are effectively heritable to subsequent bacterial cell lines, which would make such a system useful as the computational basis for many higher-order genetic devices from bacterial calculators to engineering of new metabolic pathways to bacterial drug delivery systems. | ||
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===Background=== | ===Background=== | ||
====Recombination==== | ====Recombination==== | ||
Revision as of 19:14, 25 September 2010
Description
Abstract
Sequential logic switches are the basis for many common electronic devices such as digital clocks and calculators. Here we present a novel design for the imitation of sequential logic using basic genetic parts within E. Coli. By using a combination of DNA recombinase enzymes, promoter systems, and an innovative pattern of recombinase binding sites, we can reproduce sequential-logical functions on the compact molecular scale. By using single DNA molecules as a medium for such functions within bacterial vehicles, we can essentially mimic the functionality of a combination lock, and produce a "locked" gene which can be effectively "unlocked" only after a specific sequence of inputs detected by the bacterial promoter system. Since the DNA molecule is used as a logical medium, the "locked" and "unlocked" states are effectively heritable to subsequent bacterial cell lines, which would make such a system useful as the computational basis for many higher-order genetic devices from bacterial calculators to engineering of new metabolic pathways to bacterial drug delivery systems.
Background
Recombination
Two-plasmid System
