"
Team:Warsaw/Calendar-Stage1/16 July 2010
From 2010.igem.org
(Difference between revisions)
Olchowik (Talk | contribs)
(New page: <html> <script type="text/javascript"> ActiveTab='WetLab'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <!-- do not edit above me! --> <br>16.07.2010 Friday ...)
Newer edit →
(New page: <html> <script type="text/javascript"> ActiveTab='WetLab'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <!-- do not edit above me! --> <br>16.07.2010 Friday ...)
Newer edit →
Revision as of 20:08, 16 July 2010
16.07.2010 Friday
Cloning CommunityRBSes-GFPterminator behind J23100 [promotor]
1. EcoRI/Pst digest of obtained clones to check size of the constructs
J23100-B0030-GFPterminator sample 1
J23100-B0031-GFPterminator sample 1
J23100-B0032-GFPterminator sample 1
J23100-B0033-GFPterminator sample 1
J23100-B0034-GFPterminator sample 1
2. GEL
3. Set up liquid culture of ‘cloning intermediates’ communityRBSes-GFPterminator to prepare stocks; samples:
RBS+GFP 10.1 F zag
RBS+GFP 7.2G zag
RBS+GFP 11.1P zag
RBS+GFP 10.1F
RBS+GFP 11.1P
RBS+GFP 8.1E
RBS+GFP 9.1zag
RBS+GFP 9.1A
RBS+GFP 8.1Ezag
YFP1
YFP2
CFP1
CFP2
