INSA-Lyon/14 October 2010
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Revision as of 20:17, 25 October 2010
October 14th
Transformation of the ligation of the 12/10 (with purified DNA)
Liquid culture of the ligation of the 12/10 (with unpurified DNA) :
- phasin/phasin-intein : 4 clones
- phasin-intein/2P: 7 clones
- phaC/12/13 and Curli/phaC: too much clones, the ligation doesn't success
- 18A/L2/1N : no clones
Digestion of the clones with X+P
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