"
Team:Uppsala-SwedenWeek4
From 2010.igem.org
(→Week-4) |
(→Week-4) |
||
| Line 3: | Line 3: | ||
We studied the below articles for getting an understanding of how to achieve our goals. Having studied each of the articles we had extensive discussions on "Google Wave" and during our off-line group meetings. | We studied the below articles for getting an understanding of how to achieve our goals. Having studied each of the articles we had extensive discussions on "Google Wave" and during our off-line group meetings. | ||
| + | |||
| + | Initially the idea was along the lines of obtaining band detects as done by Basu etal. However, we realised that the approach used by Basu etal, would only work for AHL. If we or another iGEM team wanted to used some other sensor they would have to again produce mutants of the sensory promoter. These mutants would respond to different concentrations of the input signal. Moreover, we realised that few of these AHL detection sensor mutants where not readily available in the registry. | ||
| + | |||
| + | The brilliant approach used by the iGEM team from Cambridge last year(2009) seemed to hold the answer for our problems. Instead of depending on the sensor for measuring the signal, a separate set of elements would do the part of measurement. However, a linear relationship between the sensor and the measuring element would be necessary. We decided to combine the idea used by Basu etal and the 2009 iGEM team from Cambridge using the parts available to us in the registry. | ||
| + | |||
| + | An inverter was readily available for us to invert the signal. This inverter is used in combination with a measuring element which gets activated at a lower level of sensory input. As soon as the input signal is strong enough to activate the inverter, the reporter inhibitor is deactivated. The higher level of the sensory input directly controls another reporter inhibitor. | ||
| + | |||
An article about the design of toggle switches and an oscillator, later used for pattern generation | An article about the design of toggle switches and an oscillator, later used for pattern generation | ||
Revision as of 14:38, 14 October 2010
Week-4
We studied the below articles for getting an understanding of how to achieve our goals. Having studied each of the articles we had extensive discussions on "Google Wave" and during our off-line group meetings.
Initially the idea was along the lines of obtaining band detects as done by Basu etal. However, we realised that the approach used by Basu etal, would only work for AHL. If we or another iGEM team wanted to used some other sensor they would have to again produce mutants of the sensory promoter. These mutants would respond to different concentrations of the input signal. Moreover, we realised that few of these AHL detection sensor mutants where not readily available in the registry.
The brilliant approach used by the iGEM team from Cambridge last year(2009) seemed to hold the answer for our problems. Instead of depending on the sensor for measuring the signal, a separate set of elements would do the part of measurement. However, a linear relationship between the sensor and the measuring element would be necessary. We decided to combine the idea used by Basu etal and the 2009 iGEM team from Cambridge using the parts available to us in the registry.
An inverter was readily available for us to invert the signal. This inverter is used in combination with a measuring element which gets activated at a lower level of sensory input. As soon as the input signal is strong enough to activate the inverter, the reporter inhibitor is deactivated. The higher level of the sensory input directly controls another reporter inhibitor.
An article about the design of toggle switches and an oscillator, later used for pattern generation
1) Danino etal 2010 (http://www.ncbi.nlm.nih.gov/pubmed/20090747)
A general paper about synthetic biology for better understanding of what we were getting into
2) Mukherji etal 2009 (http://www.ncbi.nlm.nih.gov/pubmed/19898500)
The papers about quorum sensing using AHL for pattern generation. These two papers played a major role in driving our project.
3) Basu etal (http://www.ncbi.nlm.nih.gov/pubmed/15858574 and http://www.ncbi.nlm.nih.gov/sites/pubmed/15096621)
4) Original paper about toggle switches (http://www.ncbi.nlm.nih.gov/pubmed/10659857)
5) For degrdation of color proteins -ex:carotenoids (http://www.jbc.org/content/281/15/9845.full). We planned to degrade the colors generated by the clock display unit after displaying the time for a short duration.
Having studied the articles we decided to make use of the band detect concept introduced in Basu etal to send and detect the signals between adjacent colonies without being affected by the noise from other colonies.
