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From 2010.igem.org

→ Transformed BL21 cells

- 9 samples: E1-5b, J23106, positive and negative controls

-Pipetted 100uL of BL21 cells into each centrifuge tube (x9)

-Added 1uL of DNA to all tubes except negative control. For positive control, added pUC control DNA

-Kept all tubes on ice for ~30 min(+)

-Heat shocked them with a hot water bath (30s at 42 C)

-Put on ice for another 5min

-Added 899uL LB to each--> made 1mL of solution in each tube

-Put in shaker for 1hour at 37 C

-warmed up 3 Amp plates (1 left--> need to make more)

-plated each sample on 1/3 of a plate (25uL each--> just one amount)

-put them into the incubator → 37 C overnight.