Team:Virginia United/Parts

From 2010.igem.org

igem


Parts

Biobrick Contributions

Hybrid Promoters

Ptet/ara6 Ptet/lac6 Plac/ara6 Ptet/ara110 Ptet/lac110

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References:

Tet:

1. Lutz R and Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 1997 Mar 15; 25(6) 1203-10. pmid:9092630. PubMed HubMed [Lutz-1997]

Lac:

1. Gilbert, Walter; Maxam, Allen. (1973). The Neculeotide Sequence of the lac Operator. Proc. Natl. Acad. Sci. USA. 70(12):3581-84

Ara:

1. Schlief, R. (2000). Regulation of the L-arabinose operon of Escherichia coli. Trends in Genetics. 16(12):559-565.

2. Khlebnikov A, Datsenko KA, Skaug T, Wanner BL, and Keasling JD. (2001). Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology. 147(12):3241-7.