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Contents 1 Lab-Notebook 2 DNA-multiplication 2.1 CMV-Promoter from BBJ52034 2.1.1 Work 2.1.2 Progress 2.2 eGFP 2.2.1 Work 2.2.2 Progress 3 PCR-steps 3.1 mutagenesis 3.2 fusion 4 cloning 5 insert in eukaryotic genom

Lab-Notebook

This notebook is used to structure our work and progress

DNA-multiplication

It is necessary to increase the amount of DNA we have got in order to work properly with it.

CMV-Promoter from BBJ52034

Work

We have to proliferate E. colis containing this plasmid and then extract the plasmid from the bacteria.

Progress

8/9+10 Proliferation done by Corinna, Laura, Alex, Julia

8/11 Planned extraction by Jara, Emanuel

eGFP

This Biobrick is needed several times.

Work

It has to be ...(?)

Progress

PCR-steps

..

mutagenesis

...

fusion

...

cloning

fusions

insert in eukaryotic genom

if there is time left