Team:TU Delft/Parts/characterization

From 2010.igem.org

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===Favorite Parts===
===Favorite Parts===
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====[http://partsregistry.org/Part:BBa_K398029 BBa_K398029]====
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[[Image:TUDelftALDH_final.jpg|600px]]
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Our results suggest that the recombinant strain ''E. coli'' 029A functionally express our biobrick [http://partsregistry.org/Part:BBa_K398029 BBa_K398029]. From the statistical analysis that we performed we concluded that the expression of the biobrick [http://partsregistry.org/Part:BBa_K398006 BBa_K398006] under the promoter-rbs combination [http://partsregistry.org/Part:BBa_J13002 BBa_J23100]-[http://partsregistry.org/Part:BBa_J13002 BBa_J61117] increases the dodecanal dehydrogenase activity in ''E. coli'' cell extracts 2-fold. Moreover, the enzymatic activities measured for the construct [http://partsregistry.org/Part:BBa_K398029 BBa_K398029] were equivalent to 33.98% of the ''Pseudomonas putida'' aldehyde dehydrogenase activity. 
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Read more about it in [https://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation/results our results page]
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====BBa_K398206====
====BBa_K398206====
[[Image:TU_Delft_Emuls_assay_exp1.jpg|thumb|400px|right|Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the [[Team:TU_Delft/Project/solubility/results|BBa_K398206 characteristics]]]]This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our [[Team:TU_Delft/Project/solubility|solubility]] subproject.
[[Image:TU_Delft_Emuls_assay_exp1.jpg|thumb|400px|right|Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the [[Team:TU_Delft/Project/solubility/results|BBa_K398206 characteristics]]]]This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our [[Team:TU_Delft/Project/solubility|solubility]] subproject.

Revision as of 01:38, 27 October 2010

Characterization

Our favorite parts were also the ones best characterized. This page is a quick overview of the results.

Favorite Parts

[http://partsregistry.org/Part:BBa_K398029 BBa_K398029]

TUDelftALDH final.jpg

Our results suggest that the recombinant strain E. coli 029A functionally express our biobrick [http://partsregistry.org/Part:BBa_K398029 BBa_K398029]. From the statistical analysis that we performed we concluded that the expression of the biobrick [http://partsregistry.org/Part:BBa_K398006 BBa_K398006] under the promoter-rbs combination [http://partsregistry.org/Part:BBa_J13002 BBa_J23100]-[http://partsregistry.org/Part:BBa_J13002 BBa_J61117] increases the dodecanal dehydrogenase activity in E. coli cell extracts 2-fold. Moreover, the enzymatic activities measured for the construct [http://partsregistry.org/Part:BBa_K398029 BBa_K398029] were equivalent to 33.98% of the Pseudomonas putida aldehyde dehydrogenase activity. Read more about it in our results page

BBa_K398206

Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the BBa_K398206 characteristics
This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our solubility subproject.

View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398206 BBa_K398206 in the parts registry]

Specified Components <partinfo>K398206 SpecifiedComponents</partinfo>

Designer: <partinfo>K398206 Designer</partinfo>

Status: <partinfo>K398206 Status</partinfo>