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From 2010.igem.org

Revision as of 23:10, 24 June 2010 (view source) Gcxmatt (Talk | contribs) (→DNA) ← Older edit		Revision as of 23:10, 24 June 2010 (view source) Gcxmatt (Talk | contribs) (→Bacterial Work) Newer edit →
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	===[[Kit to Stock Plasmid]]===		===[[Kit to Stock Plasmid]]===
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	===[[Transformation]]===		===[[Transformation]]===

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Revision as of 23:10, 24 June 2010

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Protocol

http://ginkgobioworks.com/support/

Formatting: http://meta.wikimedia.org/wiki/Help:Wikitext_examples

OpenWetWare Protocols: http://openwetware.org/wiki/Protocols

Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 Assembly 3.1 Restriction Enzyme Digests 3.2 3A Assembly 3.3 Ligations 3.4 Mini Prep

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep