Team:Northwestern/Project/Chitin Synthesis

From 2010.igem.org

Revision as of 19:17, 23 October 2010 by Timsterxox (Talk | contribs)


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Modeling Chassis Induction Chitin Apoptosis

Chitin is an abundant biopolymer found primarily in the exoskeletons of arthropods, including many insects and crustaceans. It is composed of N-acetylglucosamine monomers (Fig. 1)

alt text
Figure 1 Chitin molecular structure.
Source: [http://en.wikipedia.org/wiki/Chitin Wikipedia - Chitin]

- chitin - what is it, how is it used in nature - The production of chitin in yeast, pathway, mechanism

Chitin Synthase 3 (CHS3) was cloned out of Saccharomyces Cerevisiae (S.C.) cDNA.

CHS3 from S.C. was chosen because the the protein does not require cofactors or activation factors and also because it was determined to be the most active of the Chitin Synthase family.

Chitin Synthase polymerizes N-Acetyl-D-Glucosamine, also known as Chitin, with substrate as UDP-N-Acetyl-D-Glucosamine.