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Team:Newcastle/Meetings/21 October 2010
From 2010.igem.org
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| - | Apologies - Phil, Younus, Deena late | + | *Apologies - Phil, Younus, Deena late |
| - | + | *Action point: Phil and Rachel add characteristation data to the wiki | |
| - | Action point: Phil and Rachel add characteristation data to the wiki | + | |
==''yneA'' Characteristaion:== | ==''yneA'' Characteristaion:== | ||
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*Δ''amyE'':Pspac(hy)::''yneA'' | *Δ''amyE'':Pspac(hy)::''yneA'' | ||
| - | *'' | + | *''B. subtilis'' spread of IPTG may not be homogenous through the population. |
| - | *''E.coli'' has transporter deltions: check ''E.coli'' wiki | + | *''E. coli'' has transporter deltions: check ''E. coli'' wiki |
| - | take out pMap65 results | + | *take out pMap65 results |
| - | Median, Mode add to graph | + | *Median, Mode add to graph |
| - | pMutin4 proportion of cells | + | *pMutin4 proportion of cells |
| - | 20% responding- looks like | + | *20% responding- looks like |
| - | Biomass of cells - length * amount (counts)) against IPTG | + | *Biomass of cells - length * amount (counts)) against IPTG |
| - | Lysis acts to counter the overexpression of yneA ... so we have fewer cells | + | *Lysis acts to counter the overexpression of yneA ... so we have fewer cells |
| - | Younus AP: send script for yneA to Jen, Matt, Neil | + | *Younus AP: send script for yneA to Jen, Matt, Neil |
| - | Plot: Max cell length against ITPG + Error bars | + | *Plot: Max cell length against ITPG + Error bars |
==Arabinose== | ==Arabinose== | ||
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==Concrete== | ==Concrete== | ||
| - | Damaged building- earthquake | + | * Damaged building - earthquake |
==Flux balance== | ==Flux balance== | ||
| - | difference between optimal and normal | + | *difference between optimal and normal |
==Lab work== | ==Lab work== | ||
Revision as of 16:17, 25 October 2010
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- Apologies - Phil, Younus, Deena late
- Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
We think we have it cloned infront of GFP Bacillus subtilis transformation tommorrow Fluorescence Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
DNA submitted... tracking number sent 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
Deena and Harsh
Risk assessment- Bacillus
BioCOSH
Safety Guy talk mention it
Presentation
Phil DON'T BE AGGRESSIVE!!!!
Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- Previous work on CaCO3
- Don't use the word PROVES
- Separate slid for work flows
- Alan - Flux first, speak slowly
- Summary table- redo
- BRONZE, SILVER, GOLD!!!!!!! (see below)
GOLD
Jannetta write RFC
- number on judging form
- webservice!!!
SEM
Images are ours
Other
Insurance form Taxi
Younus - Poster next thursday due in?
Action point check presentation and poster submission!!!
   
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