Team:Newcastle/Meetings/1 July 2010

From 2010.igem.org

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==Roll call==
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==Formal Meeting - 1st July 2010==
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Everyone here except Phil and Younus who are in the lab. Arrived 10mins later.
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==Action points from last week==
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*[[Team:Newcastle/Agendas/1_July_2010#Formal_Meeting_-_1st_July_2010| Agenda]]
 +
====Roll call====
 +
Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.
 +
 
 +
====Action points from last week====
* Wiki changes have been made.
* Wiki changes have been made.
* Letter to Andrew Young is not finished - someone needs to be in charge of this.
* Letter to Andrew Young is not finished - someone needs to be in charge of this.
* Civil engineer funding is not done yet.
* Civil engineer funding is not done yet.
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* Flights: plan is to get there on Thursday about 4-5pm.
+
* Flights: plan is to get there on Thursday about 4-5 p.m.
-
==Research feedback - end of crack signalling, quorum sensing==
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====Research feedback - end of crack signalling, quorum sensing====
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--Diagram--
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* Oxygen limitation triggers subtilin production, ''ResD'' and ''E''.
* Oxygen limitation triggers subtilin production, ''ResD'' and ''E''.
* Need subtilin immunity brick too ''spaIFEG''.
* Need subtilin immunity brick too ''spaIFEG''.
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* We need to get the strain with these genes. ATCC6633.
* We need to get the strain with these genes. ATCC6633.
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==Lab feedback==
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====Lab feedback====
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Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4.
+
*Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
-
Urea media, grown overnight, If the urea is used, there will be a colour change.
+
*Urea media, grown overnight, If the urea is used, there will be a colour change.
-
Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
+
*Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
-
Phil and Younus, transforming ''E. coli'' dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.
+
*Phil and Younus, transforming ''E. coli'' dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.
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==Logo and T-shirts==
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====Logo and T-shirts====
* Finalised.
* Finalised.
* University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
* University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
* CBCB logo needs to be added. Ask Jeff or Joanna.
* CBCB logo needs to be added. Ask Jeff or Joanna.
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==Visas==
+
====Visas====
* Younus still hasn't filled in the visa-waiver form.
* Younus still hasn't filled in the visa-waiver form.
-
==Funding==
+
====Funding====
* Andrew Young letter needs to be finished and checked tomorrow.
* Andrew Young letter needs to be finished and checked tomorrow.
-
==Timeline==
+
====Timeline====
* Could be made more detailed now.
* Could be made more detailed now.
* Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
* Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
-
* Spider silk next? It's on our logo so we should do it..
+
* Spider silk next? It's on our logo so we should do it.
-
==Action points==
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====Action points====
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* Phil: talk to Dr. Aldridge about glue. How easy would a glue brick be?
+
* Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
* All: Andrew Young letter needs to be finished and checked tomorrow.
* All: Andrew Young letter needs to be finished and checked tomorrow.
-
==Next meeting==
+
====Next meeting====
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Harsh is chair, Phil is computer, Alan in minutes. Meeting is 3pm Wednesday.
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*Next Wednesday, 3 p.m.
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*Chair: Harsh, Minutes: Alan, Computer: Phil
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 01:38, 26 October 2010

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Contents

Formal Meeting - 1st July 2010

Roll call

Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.

Action points from last week

  • Wiki changes have been made.
  • Letter to Andrew Young is not finished - someone needs to be in charge of this.
  • Civil engineer funding is not done yet.
  • Flights: plan is to get there on Thursday about 4-5 p.m.

Research feedback - end of crack signalling, quorum sensing

  • Oxygen limitation triggers subtilin production, ResD and E.
  • Need subtilin immunity brick too spaIFEG.
  • Subtilin receptor was done in 2008 by Newcastle spaRK.
  • Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
  • We need to get the strain with these genes. ATCC6633.

Lab feedback

  • Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
  • Urea media, grown overnight, If the urea is used, there will be a colour change.
  • Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
  • Phil and Younus, transforming E. coli dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.

Logo and T-shirts

  • Finalised.
  • University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
  • CBCB logo needs to be added. Ask Jeff or Joanna.

Visas

  • Younus still hasn't filled in the visa-waiver form.

Funding

  • Andrew Young letter needs to be finished and checked tomorrow.

Timeline

  • Could be made more detailed now.
  • Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
  • Spider silk next? It's on our logo so we should do it.

Action points

  • Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
  • All: Andrew Young letter needs to be finished and checked tomorrow.

Next meeting

  • Next Wednesday, 3 p.m.
  • Chair: Harsh, Minutes: Alan, Computer: Phil
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