Team:Newcastle/Meetings/1 July 2010

From 2010.igem.org

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(New page: ==Roll call== Everyone here except Phil and Younas who are in the lab. ==Action points from last week== Wiki changes done. Letter to Andrew Young is not - someone needs to be in charge of...)
 
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==Roll call==
+
{{Team:Newcastle/mainbanner}}
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Everyone here except Phil and Younas who are in the lab.
+
==Formal Meeting - 1st July 2010==
-
==Action points from last week==
+
*[[Team:Newcastle/Agendas/1_July_2010#Formal_Meeting_-_1st_July_2010| Agenda]]
-
Wiki changes done.
+
====Roll call====
-
Letter to Andrew Young is not - someone needs to be in charge of this.
+
Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.
-
Civil engineer funding is not done yet.
+
 
-
Flights plan is to get there on thursday about 4-5pm.
+
====Action points from last week====
-
==Reseach feedback - end of crack signalling, quorum sensing==
+
* Wiki changes have been made.
-
Diagram on board.
+
* Letter to Andrew Young is not finished - someone needs to be in charge of this.
-
Oxygen limitation triggers subtilin production, Res D and E. Need subtilin immunity brick too spaIFEG. Subtilin receptor was done in 2008 by Newcastle spaRK.
+
* Civil engineer funding is not done yet.
 +
* Flights: plan is to get there on Thursday about 4-5 p.m.
 +
 
 +
====Research feedback - end of crack signalling, quorum sensing====
 +
* Oxygen limitation triggers subtilin production, ''ResD'' and ''E''.
 +
* Need subtilin immunity brick too ''spaIFEG''.
 +
* Subtilin receptor was done in 2008 by Newcastle ''spaRK''.
 +
* Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
 +
* We need to get the strain with these genes. ATCC6633.
 +
 
 +
====Lab feedback====
 +
*Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
 +
*Urea media, grown overnight, If the urea is used, there will be a colour change.
 +
 
 +
*Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
 +
 
 +
*Phil and Younus, transforming ''E. coli'' dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.
 +
 
 +
====Logo and T-shirts====
 +
* Finalised.
 +
* University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
 +
* CBCB logo needs to be added. Ask Jeff or Joanna.
 +
 
 +
====Visas====
 +
* Younus still hasn't filled in the visa-waiver form.
 +
 
 +
====Funding====
 +
* Andrew Young letter needs to be finished and checked tomorrow.
 +
 
 +
====Timeline====
 +
* Could be made more detailed now.
 +
* Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
 +
* Spider silk next? It's on our logo so we should do it.
 +
 
 +
====Action points====
 +
* Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
 +
* All: Andrew Young letter needs to be finished and checked tomorrow.
 +
 
 +
====Next meeting====
 +
*Next Wednesday, 3 p.m.
 +
*Chair: Harsh, Minutes: Alan, Computer: Phil
 +
{{Team:Newcastle/footer}}

Latest revision as of 01:38, 26 October 2010

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Contents

Formal Meeting - 1st July 2010

Roll call

Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.

Action points from last week

  • Wiki changes have been made.
  • Letter to Andrew Young is not finished - someone needs to be in charge of this.
  • Civil engineer funding is not done yet.
  • Flights: plan is to get there on Thursday about 4-5 p.m.

Research feedback - end of crack signalling, quorum sensing

  • Oxygen limitation triggers subtilin production, ResD and E.
  • Need subtilin immunity brick too spaIFEG.
  • Subtilin receptor was done in 2008 by Newcastle spaRK.
  • Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
  • We need to get the strain with these genes. ATCC6633.

Lab feedback

  • Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
  • Urea media, grown overnight, If the urea is used, there will be a colour change.
  • Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
  • Phil and Younus, transforming E. coli dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.

Logo and T-shirts

  • Finalised.
  • University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
  • CBCB logo needs to be added. Ask Jeff or Joanna.

Visas

  • Younus still hasn't filled in the visa-waiver form.

Funding

  • Andrew Young letter needs to be finished and checked tomorrow.

Timeline

  • Could be made more detailed now.
  • Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
  • Spider silk next? It's on our logo so we should do it.

Action points

  • Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
  • All: Andrew Young letter needs to be finished and checked tomorrow.

Next meeting

  • Next Wednesday, 3 p.m.
  • Chair: Harsh, Minutes: Alan, Computer: Phil
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