Team:Newcastle

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=BacillaFilla: Fixing Cracks in Concrete=
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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
 
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[[Image:Newcastle_iGEM_Teampic.jpeg|centre|468px]]
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===Project Description===
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BacillaFilla, an engineered ''Bacillus subtilis'', aims to repair [[Team:Newcastle/problem|cracks in concrete]] which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surfaces.
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BacillaFilla would swim deep into the cracks. Repair would be effected by production of [[Team:Newcastle/Urease|CaCO<sub>3</sub>]], [[Team:Newcastle/Filamentous_Cells|filamentous ''B. subtilis'' cells]] and [[Team:Newcastle/glue|levansucrose glue]]. CaCO<sub>3</sub> expands at the same rate as concrete, making it an ideal filler. Filamentous ''B. subtilis'' cells have similar tensile strength to the synthetic fibres used in fibre-reinforced concrete, and provide reinforcement. Levansucrose glues CaCO<sub>3</sub> and filamentous cells in place.
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|You can write a background of your team here. Give us a background of your team, the members, etcOr tell us more about something of your choosing.
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|[[Image:Newcastle_logo.png|200px|right|frame]]
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''B. subtilis'' 168 sporulates, making it ideal for storage and transportation. The cells can be [[Team:Newcastle/solution#Alkalinity_resistance|made to be tolerant to concrete's high pH]].
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We designed a [[Team:Newcastle/Swarming|swarming]] BioBrick part for repairing ''B. subtilis'' 168's defective ''swrA'' and ''sfp'' genes, regaining motility. At the end of the crack the quorum sensing peptide [[Team:Newcastle/End_of_crack_%26_signalling_system|subtilin]] triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter]]. Upregulating ''SR1'' and ''rocF'' promotes arginine and urea production, increasing exogenous CaCO<sub>3</sub> deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levan glue.
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To protect the environment our project also includes a design for a [[Team:Newcastle/Non-target-environment_kill_switch| kill switch]].
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Our project is summarised in the diagram below:
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[[Image:newcastle_summary.png|600px|centre]]
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===Summary of Our Achievements===
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|<span style="color:Sienna">'''BRONZE:'''</span>
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''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|From the beginning of the project, we have successfully registered the team of two instructors, six advisors and eight members. We have completed and submitted a Project Summary form, developed ideas and shared them on our iGEM wikipedia page. We also entered 31 new BioBrick parts into the Registry of Parts. One part, [http://partsregistry.org/Part:BBa_K302012 IPTG-induced filamentous cell formation], the BioBrick for the IPTG-induced filamentous cell formation, was demonstrated to work as expected.
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|[[Image:Newcastle_team.png|right|frame|Your team picture]]
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|<span style="color:Silver">'''SILVER:'''</span>
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|Our new BioBrick, the IPTG-inducible filamentous cell formation part works, so we [[Team:Newcastle/Filamentous_Cells#Characterisation|characterised]] it and included the information, [http://partsregistry.org/Part:BBa_K302012:Experience BBa_K302012], on the Parts Registry.
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|<span style="color:Goldenrod">'''GOLD:'''</span>
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|In order to obtain a gold, we investigated the benefits of an e-Science approach, focusing on workflows, to synthetic biology. Details can be found at [[Team:Newcastle/E-Science|here]]. This part of the project resulted in us proposing a new standard for a RESTful API which facilitates the discovery and publication of models of functional biological units. The standard has been submitted to the BioBricks Foundation as [[BBFRFC66|BBF RFC 66]].
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|align="center"|[[Team:Newcastle | Team Example]]
 
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<!---[http://twitter.com/newcastle_igem Follow us on Twitter] or join our [http://www.facebook.com/pages/Newcastle-iGEM-2010/140948965930577| Facebook Fan page!] --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="2" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
!align="center"|[[Team:Newcastle|Home]]
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!align="center"|[[Team:Newcastle/Team|Team]]
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!align="center"|[[Team:Newcastle/Project|Project]]
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!align="center"|[[Team:Newcastle/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Newcastle/Modelling|Modelling]]
!align="center"|[[Team:Newcastle/Notebook|Notebook]]
!align="center"|[[Team:Newcastle/Notebook|Notebook]]
!align="center"|[[Team:Newcastle/Safety|Safety]]
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Latest revision as of 19:13, 25 November 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

BacillaFilla: Fixing Cracks in Concrete

Newcastle iGEM Teampic.jpeg

Project Description

BacillaFilla, an engineered Bacillus subtilis, aims to repair cracks in concrete which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surfaces.

BacillaFilla would swim deep into the cracks. Repair would be effected by production of CaCO3, filamentous B. subtilis cells and levansucrose glue. CaCO3 expands at the same rate as concrete, making it an ideal filler. Filamentous B. subtilis cells have similar tensile strength to the synthetic fibres used in fibre-reinforced concrete, and provide reinforcement. Levansucrose glues CaCO3 and filamentous cells in place.

B. subtilis 168 sporulates, making it ideal for storage and transportation. The cells can be made to be tolerant to concrete's high pH.

We designed a swarming BioBrick part for repairing B. subtilis 168's defective swrA and sfp genes, regaining motility. At the end of the crack the quorum sensing peptide subtilin triggers a co-ordinated population response from a subtilin-inducible promoter. Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous CaCO3 deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levan glue.

To protect the environment our project also includes a design for a kill switch.

Our project is summarised in the diagram below:

Newcastle summary.png


Summary of Our Achievements

BRONZE: From the beginning of the project, we have successfully registered the team of two instructors, six advisors and eight members. We have completed and submitted a Project Summary form, developed ideas and shared them on our iGEM wikipedia page. We also entered 31 new BioBrick parts into the Registry of Parts. One part, [http://partsregistry.org/Part:BBa_K302012 IPTG-induced filamentous cell formation], the BioBrick for the IPTG-induced filamentous cell formation, was demonstrated to work as expected.
SILVER: Our new BioBrick, the IPTG-inducible filamentous cell formation part works, so we characterised it and included the information, [http://partsregistry.org/Part:BBa_K302012:Experience BBa_K302012], on the Parts Registry.
GOLD: In order to obtain a gold, we investigated the benefits of an e-Science approach, focusing on workflows, to synthetic biology. Details can be found at here. This part of the project resulted in us proposing a new standard for a RESTful API which facilitates the discovery and publication of models of functional biological units. The standard has been submitted to the BioBricks Foundation as BBF RFC 66.


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
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