Team:LMU-Munich/Notebook/Protocols/12 Gel extraction or PCR Clean up
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Source:http://www.biodee.net/UploadFile/Up-2009-11-26_633948249752287808-a9281.pdf | Source:http://www.biodee.net/UploadFile/Up-2009-11-26_633948249752287808-a9281.pdf | ||
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+ | Note: from 10ng to 40µg of DNA can be bound by the membrane (don't try to eluate more, as membrane could be blocked) | ||
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2. Add 10μl Membrane Binding Solution per 10mg of gel slice. Vortex and | 2. Add 10μl Membrane Binding Solution per 10mg of gel slice. Vortex and | ||
- | incubate at 50–65°C until gel slice is completely dissolved. | + | incubate at 50–65°C (we used 95°C as the agar didn't melt at 60°C) until gel slice is completely dissolved. |
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<b>Elution</b> | <b>Elution</b> | ||
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7. Carefully transfer Minicolumn to a clean 1.5ml microcentrifuge tube. | 7. Carefully transfer Minicolumn to a clean 1.5ml microcentrifuge tube. |
Latest revision as of 13:40, 19 August 2010