Team:Imperial College London/Software Tool

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Revision as of 10:37, 20 October 2010

Department of Bioengineering

Division of Molecular Biosciences

Software Tool

We realised early on that our detection module could be designed with a sensitivity to different proteases. By changing the cleavage site the system can accept a wide variety of inputs. This tool is designed to facilitate a quick custom sequence generation of the entire surface protein construct.

Select Protease		Description
		This was our primary target. Read our wiki to find out more!
Awaiting sequence generation...
Yellow - Biobrick Prefix/Suffix Orange - Promoter Red - Ribosome Binding Site Purple - Cell Wall Binding Domain Dark Blue - Adjustable Linker Light Blue - Protease Cleavage Site Dark Green - Autoinducing Peptide Light Green - Terminator

@@ Line 24: / Line 24: @@
    var rbs = "TACTAGAGTCTTCAGAAAGGAGT";
    var cwb = "ATGCGTTCTTATATAAAAGTCCTAACAATGTGTTTTCTGGGGCTCATACTTTTTGTGCCAAC AGCTTTGGCCGATAACTCAGTGAAAAGAGTTGGGGGAAGCAATAGATACGGCACTGCTGTACAAATATCAAAGCAAATGTATTCAA CAGCAAGTACAGCTGTAATTGTTGGTGGGAGTTCCTATGCAGATGCTATTTCAGCAGCACCTCTTGCTTACCAGAAGAATGCGCCA TTGCTTTACACTAATTCTGATAAGCTTTCATATGAAACGAAAACAAGATTGAAAGAGATGCAGACTAAAAATGTAATTATTGTAGG CGGAACACCTGCTGTTTCTTCTAACACTGCTAACCAGATTAAAAGCTTGGGGATAAGTATTAAACGAATTGCAGGAAGCAACCGTT ATGATACGGCTGCACGGGTGGCAAAAGCGATGGGTGCGACTTCAAAAGCTGTTATTTTGAACGGCTTCTTATATGCAGACGCTCCG GCCGTCATCCCTTATGCAGCGAAAAACGGGTATCCAATTCTTTTTACAAATAAAACATCTATAAATAGTGCGACTACGTCTGTGAT AAAAGATAAGGGAATTTCGAGTACCGTTGTTGTAGGAGGCACTGGAAGTATCAGCAATACGGTATACAACAAGTTACCTTCTCCTA CAAGAATTAGCGGTTCAAACAGATATGAGCTTGCTGCAAATATCGTACAAAAACTTAATTTATCAACAAGCACCGTATATGTAAGC AATGGATTCAGCTACCCTGACTCTATTGCAGGAGCTACACTGGCAGCTAAGAAGAAGCAATCTCTTATTCTTACAAATGGTGAAAA TTTATCTACAGGAGCCCGTAAAATTATTGGAAGTAAAAACATGTCAAACTTTATGATTATCGGAAACACTCCTGCCGTAAGCACAA AGGTTGCTAATCAGCTAAAGAATCCAGTTGTA";
-   var linker = "AGCAGGGGTAGCAGGGCTGGTGGCGGTGGCGGTGGC";
+   var linker = "AGCAGGGGTAGCAGGGCT GGTGGCGGTGGCGGTGGC";
    var cleavage = new Array();
    cleavage[1] = "AGCTGGCCTCTT";
@@ Line 31: / Line 31: @@
    cleavage[4] = "ATCGACGGACGT";
    cleavage[5] = "blank";
-   var aip = "GAA ATGCGCCTTAGCAAATTCTTCAGGGACTTCATTCTTCAAAGGAAAAAA";
+   var aip = "GAAATGCGCCTTAGCAAATTCTTCAGGGACTTCATTCTT CAAAGGAAAAAA";
    var terminator = "TAATAA";
    var suffix = "blank";