Team:GeorgiaTech/Notebook

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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The purpose of the proposed research is to genetically engineer bacteria to generate large amounts of heat in response to a cold shock. This will advance biological engineering by furthering current attempts at constructing and understanding complex gene networks. Applications of synthetic, thermogenerating bacteria range from the investigation of cold resistance evolution to the development of a new generation of inexpensive biosensors for environmental monitoring and contaminant detection.  The experiment will focus on producing two distinct responses to a decrease in ambient temperature: cell aggregation leading to biofilm formation and overexpression of alternative oxidase (AOX), an enzyme associated with thermogenesis in plants. </p>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The purpose of the proposed research is to genetically engineer bacteria to generate large amounts of heat in response to a cold shock. Applications of synthetic, thermogenerating bacteria range from aiding in evolutionary comprehension by modifying an organism’s response to an external environment to in vitro optimization of protein direction.</p>
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<p>Microorganisms respond to environmental stress through several means for survival.  This research project also aims to engineer Escherichia coli cells to aggregate with one another forming a biofilm given a cold shock and link this “huddle” response with heat generation. Altering a cell’s reaction to external variations prolongs its ability to survive such a shock, thereby ensuring an evolutionary advantage over other species. Studying and manipulating a prokaryote’s stress response can broaden the prospective on evolutionary mechanisms in general.
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<p>Microorganisms respond to environmental stress through several means for survival. Altering a cell’s reaction to external variations prolongs its ability to survive such a shock, thereby ensuring an evolutionary advantage over other species. Studying and manipulating a prokaryote’s stress response can broaden the prospective on evolutionary mechanisms. This research venture will advance biological engineering by furthering current attempts at constructing and understanding complex gene networks. Applications of synthetic, thermogenerating bacteria range from the investigation of cold resistance evolution to controlling a simple organism’s output to a given input. The experiment will focus on producing two distinct responses to a decrease in ambient temperature: over-expression of alternative oxidase (AOX), an enzyme associated with thermogenesis in plants via a cold-induced promoter and
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subsequent heat generation.
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<p><center><font color="#FFFFFF" size=5><b>Weekly Logbook</b></font></center></p>
<p><center><font color="#FFFFFF" size=5><b>Weekly Logbook</b></font></center></p>
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<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/WeekEleven">Week Eleven 10/10/2010 - 10/16/2010</a></center>
<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/WeekEleven">Week Eleven 10/10/2010 - 10/16/2010</a></center>
<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/WeekTwelve">Week Twelve 10/17/2010 - 10/23/2010</a></center>
<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/WeekTwelve">Week Twelve 10/17/2010 - 10/23/2010</a></center>
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<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/WeekThirteen">Week Thirteen 10/24/2010 - 10/27/2010</a></center>
<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/Protocols">Protocols</a></center>
<p><center><a href="https://2010.igem.org/Team:GeorgiaTech/Protocols">Protocols</a></center>
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<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />
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==Notebook==
 
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
 

Latest revision as of 03:13, 28 October 2010