Team:Cambridge/Tools/Gibson

Gibthon Beta - here

The more basic of the two tools, this will take as an input the sequences surrounding the join for which you wish to design primers. You can interactively adjust the length of the primers to tune the melting temperature of the DNA and check for secondary structures.

The developer is currently focussing on GCD, but will still be supporting Gibthon - contact him here

Gibthon Construct Designer Beta - here

Gibthon's older brother, and much more powerful. With this, you can design your construct from its component genes and automatically generate the optimal primers. The tool has access to the Parts Registry Database, the NCBI's Nucleotide database, as well as accepting genbank formatted files from the user. The user is able to store constructs and their own genes for recovery later.

Generating the primers for PCR extension takes only a few seconds each, and the information is returned to the user in the form of a PDF with a datasheet for each primer. In the near future it will also provide all the necessary protocols to complete your construct. As time goes by, more granular conrol over settings will become available.

The algorithm for optimising primer length is still under development, so should not be entirely trusted until the software comes out of beta. When you find something that doesn't work, or could be improved, please email the developer.

How it works

For the latest information on what algorithms Gibthon uses for generating primers, please check the site as this wiki might be a bit behind. There is also a video demo you can watch here on how to use it: