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Team:Calgary/16 June 2010
From 2010.igem.org
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| + | Dev, Jeremy, and Chris: Today, we observed the overnight cultures and the attempted constructions that were done yesterday. Dev spent the day registering for his courses. We Miniprepped the parts K239000, J61032, and K135000 which came from the Registry. The part J61032 was deemed unusable as the quality did not meet our standards as the plasmid concentration was observed. We also received periplasmic RFP from Dr. Schryvers' lab and the overnight cultures made from it were Miniprepped. As well, overnight cultures of many other parts from the Registry were made into glycerol stocks. Dev's construct of J13002-E0040 had tons of growth on the plate, but the colonies did not appear to fluoresce. Chris's plates of J13002-E0032 and pSB1K2-J23032 showed no growth once again. Tomorrow, another construction was planned with different vectors and inserts. As well, the Suffield R & D base was contacted and a possible tour was discussed with them. | ||
Revision as of 21:28, 16 June 2010
Wednesday June 16, 2010
Dev, Jeremy, and Chris: Today, we observed the overnight cultures and the attempted constructions that were done yesterday. Dev spent the day registering for his courses. We Miniprepped the parts K239000, J61032, and K135000 which came from the Registry. The part J61032 was deemed unusable as the quality did not meet our standards as the plasmid concentration was observed. We also received periplasmic RFP from Dr. Schryvers' lab and the overnight cultures made from it were Miniprepped. As well, overnight cultures of many other parts from the Registry were made into glycerol stocks. Dev's construct of J13002-E0040 had tons of growth on the plate, but the colonies did not appear to fluoresce. Chris's plates of J13002-E0032 and pSB1K2-J23032 showed no growth once again. Tomorrow, another construction was planned with different vectors and inserts. As well, the Suffield R & D base was contacted and a possible tour was discussed with them.
