Team:Calgary/14 June 2010

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Monday June 14, 2010

Dev, Chris, and Jeremy: Today, we did the constructions of J13002 (a combination of a constitutive promoter with a RBS) and E0040 (GFP) and E0032 (YFP) to determine if the two fluorescent proteins were functional. This construction was done using standard Biobrick cloning and then transformation into Top10 Competent cells. We received the parts from the registry in the form of agar stabs on friday June 11, 2010 but they were received too late to work with them. The agar stabs were streaked onto plates and left to grow overnight of the parts I0500, K239000, K135000, and K274210.