Team:Alberta/Building Parts

From 2010.igem.org

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'''10-05-2010'''
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=10-05-2010=
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.  
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.  

Revision as of 20:22, 31 May 2010

Building Parts


May
MTWTFSS
          [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_May_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_May_2010&action=edit 2]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_May_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_May_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_May_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_May_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_May_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_May_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_May_2010&action=edit 9]
[http://2010.igem.org/Team:Alberta/10_May_2010 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_May_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_May_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_May_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_May_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_May_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_May_2010&action=edit 16]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_May_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_May_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_May_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_May_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_May_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_May_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_May_2010&action=edit 23]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_May_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_May_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_May_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_May_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_May_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_May_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_May_2010&action=edit 30]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/31_May_2010&action=edit 31]
June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_June_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_June_2010&action=edit 27]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_June_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_June_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_June_2010&action=edit 30]
July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_July_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_July_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_July_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_July_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_July_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_July_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_July_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_July_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_August_2010&action=edit 1]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_August_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_August_2010&action=edit 8]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_August_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_August_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_August_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_August_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_August_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_August_2010&action=edit 22]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_August_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_August_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_August_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_August_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_August_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/31_August_2010&action=edit 31]
September
MTWTFSS
    [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_September_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_September_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_September_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_September_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_September_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_September_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_September_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_September_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_September_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_September_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_September_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_September_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_September_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_September_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_September_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_September_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_September_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_September_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_September_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_September_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_September_2010&action=edit 30]

10-05-2010

PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.

Recipe:

1uL p1003 (approx. 1ng)
2.5uL prA_p1003+
2.5uL prB'_p1003-
5uL 10X PCR buffer
1uL 10uM dNTPs
2uL 50uM MgCl~2~
0.5uL Taq polymerase
35.5uL MilliQ H~2~O

Program: