Team:Edinburgh/Notebook/Green light sensor

From 2010.igem.org





These notes correspond to the part of the project detailed here.

Green Light Sensor



8/10/2010

  • running purified PCR: marker (lane 1), PhOR (Lane 2), Ccas (Lane 3).

148.JPG

19/10/20010

  • Lane 1: marker
  • Lane 2: PheA (very faint band or primer smear?)
  • Lane 3: Ccas + PhoR ligation

173.JPG

22/10/2010

  • PhoA + LacZ

174.JPG

23/10/2010

  • Lane 1: marker
  • Lane 2: phA promoter + lac Z
  • rest- irrelevant

175.JPG

25/10/2010

  • pCB + green light sensor

176.JPG

26/10/2010

  • total green light sensor + reporter

177.JPG

27/10/2010

  • green light sensor ligations into PSB1C3

178.JPG