Team:UT-Tokyo/Gel DNA Recovery
From 2010.igem.org
Gel DNA Recovery
Preparation
- ・Kit of promega
- ・MilliQ
- ・Gel
- ・Make sure that tank for electrophoresis and EtBr are not polluted.
Procedure
- 1. Cut out gel with wanted band and put it in a tube.
- 2. Add 3 parts Mem. binding sol. to 1 part Gel volume.
- 3. Shake & Incubate at 57 degrees C until gel melts.
- 4. Put in the column.
- 5. Centrifuge for 1 min at the largest spin number (at room temperature)
- 6. Empty collection tube and add 700 ul Mem. Wash sol, centrifuge for 1 minute.
- 7. Empty collection tube and add 500 ul Mem. Wash sol, centrifuge for 1 minute.
- 8. Change the column to a new 1.5 ml Eppendorf and centrifuge for 1 minute.
- 9. Change the old tube to a new 1.5 ml Eppendorf, add 30ul MilliQ, leave for 1 minutes, then centrifuge for 1 minutes.
- ! Use Nuclease-Free Water instead of MilliQ
- 10. Measure concentration, label the Eppendorf.
Copyright © 2010 iGEM UT-Tokyo. All rights reserved.