Thursday August 12, 2010

Chris

Today, I started with a ton of plasmid preparations with I13504, iPBAB, I0500-I13504, and I13507 colonies. The concentrations are once again, available upon demand. Then, I began a digest of ibpAB to with the enzymes EcoRI and PstI to insert it into an AK Biobrick vector. I also digested the CpxP promoter that was inserted into AK plasmid with EcoRI and PstI. The results of the gel with the digested CpxP and ipbAB are shown on the first gel on the right. The second gel was the ibpAB promoter uncut in original state. Finally, I set up another digest for ibpAB to run with T4 DNA Ligase tomorrow as we are out of Quick Ligase.