Emily's PCR Gel

Wednesday June 23, 2010

Patrick

Today, I did a restriction digest in order to construct the cpxP PCR product into pSB1A3 plasmid. This was done with XbaI and SpeI as those were the only sites on the PCR product. This will result in unintentional scars, but we will hopefully deal with this later on. Along with this, I also constructed an R0040 promoter in front of both E0430 and E0420 (switched onto pSB1K2).

Dev

10 colonies of growth were found from the transformation of the E0040 + B0015 construct from yesterday. A colony PCR was conducted with all 10 colonies. The PCR products were mixed with loading dye and water and ran on a 1% agarose gel.

Dev's Colony PCR Gel