Team:Northwestern/Protocol
From 2010.igem.org
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DNAPrepping DNA from the kit plates Prepping DNA from the kit platesQuikchange (from primers to colonies!)Kit to Stock PlasmidEthanol PrecipitationNew Part Design(PCR)Bacterial WorkTransformationO/N CulturePreparation of Competent CellsLB MediaPreparing PlatesGlycerol StocksAssemblyRestriction Enzyme DigestsPlasmid Construction3A AssemblyLigationsMini PrepMicroscopyConfocal MicroscopyFluorescence MicroscopyReagentsReagentsCell StainingRhodamine-Conjugated Chitin ProbeMethanol Fixation
LIVE/DEAD® BacLight - Bacterial Viability Kit |