Team:Northwestern/Notebook
From 2010.igem.org
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10/3/10Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel. Kevin came in and ran the digested CP-LacPI-GFP, digested CP-LacPI with digested Cp and digested LacPI parts. 10/4/10Timi and Kevin decided to take a day off :) 10/5/10Timi and Kevin came in to discuss why their gels were running strangely. We believe there is something wrong with our buffer, so we are swapping everything out with a fresh batch of Prof. Mordacq's 0.5x TBE buffer. Timi poured a large gel that we will be using tomorrow. 10/6/1010/7/10Timi- took part 2-17F out of the DNA kit. Transformed into T10 cells to grow overnight. 10/8/10Kevin- ran gel of the CP-LacPI parts. Gel results are still inconclusive. Also put in an overnight culture of the 2-17F part into the incubator for Timi. 10/9/10Timi- Miniprepped the 2-17F part. Digested the DNA with E/P and ligated the 2-17F GFP part into Chlor and Tet backbone. 10/10/10Timi- Transformed and plated part 2-17F onto Chlor and Tet plates. Will be ready for miniprepping tomorrow. 10/11/1010/12/10Matt - digested LacP-RBS with SpeI in preparation for standard assembly with ES digested CHS3 10/13/1010/14/10Kevin transformed T10 cells with CP-LacPI parts that we did not have much digest of left. He plated them onto TET plates. 10/15/10Kevin took out the CP-LacPI plates and put them into the 4 degree for Timi. Timi inoculated overnight cultures at 7pm. 10/16/10Timi and Kevin miniprepped the CP-LacPI inoculations, digested them with ___, and ligated them to GFP. |