Team:LMU-Munich/Notebook/Pathway
From 2010.igem.org
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- | <font color="#009933"> | + | <font color="#009933"> Preparing Competent Cells</font> |
with Benny's protocoll & buffers | with Benny's protocoll & buffers | ||
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- | <font color="#009933"> | + | <font color="#009933"> Test-Transformation</font> |
in order to see whether the sells will work | in order to see whether the sells will work | ||
Revision as of 15:27, 24 August 2010
Some test text in bold
We created following tests:
Example of a table
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Preparing Competent Cells
with Benny's protocoll & buffers
- grow the culture to an OD 600 of ~ 0,3
-> our cells: OD 600 = 0,256
- centrifuge for 10 min at 4°C by 3000rpm
- resuspend the pellet in buffer 1
-> we used 400 µl
- centrifuge for 10 min at 4°C by 2500rpm
- resuspend the pellet in less buffer 1 than before
-> we used 200 µl
- add buffer 2 in a ratio of 1:10 = buffer 2:buffer 1
-> we added 20µl of buffer 2
- incubate on ice for 10 min
-> as we probably used too little buffer, we added another 200 µl of buffer 1 and 20µl of buffer 2
- aliquote the suspension and shockfreeze it at -80°C
-> we put 50 µl in each eppendorf
- done with protocoll 3 Transformation
-> we tested with the following DNA:
-> as the cells are probably in a to high concentration, we thined them down with saline to an end-concentration of 10 -2
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weekend
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weekend
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Pathway Notebook
Contents
Week Days
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
31
8-02-2010
8-03-2010
8-04-2010
8-05-2010
8-06-2010
8-07-2010
8-08-2010
32
8-09-2010
8-10-2010
8-11-2010
8-12-2010
8-13-2010
8-14-2010
8-15-2010
33
8-16-2010
8-17-2010
8-18-2010
8-19-2010
8-20-2010
8-21-2010
8-22-2010
34
8-23-2010
8-24-2010
8-25-2010
8-26-2010
8-27-2010
8-28-2010
8-29-2010
35
8-30-2010
8-31-2010
9-01-2010
9-02-2010
9-03-2010
9-04-2010
9-05-2010
36
9-06-2010
9-07-2010
9-08-2010
9-09-2010
9-10-2010
9-11-2010
9-12-2010
37
9-13-2010
9-14-2010
9-15-2010
9-16-2010
9-17-2010
9-18-2010
9-19-2010
38
9-20-2010
9-21-2010
9-22-2010
9-23-2010
9-24-2010
9-25-2010
9-26-2010
39
9-27-2010
9-28-2010
9-29-2010
9-30-2010
10-01-2010
10-02-2010
10-03-2010
8-02-2010
8-03-2010
8-04-2010
header 1
header 2
header 3
row 1, cell 1
row 1, cell 2
row 1, cell 3
row 2, cell 1
row 2, cell 2
row 2, cell 3
8-05-2010
8-06-2010
8-07-2010
8-08-2010
8-09-2010
8-10-2010
8-11-2010
8-12-2010
8-13-2010
8-14-2010
8-15-2010
8-16-2010
8-17-2010
8-18-2010
8-19-2010
8-20-2010
8-21-2010
8-22-2010
8-23-2010
8-24-2010
Test-Transformation
in order to see whether the sells will work
8-25-2010
8-26-2010
8-27-2010
8-28-2010
8-29-2010
8-30-2010
8-31-2010
9-01-2010
9-02-2010
9-05-2010
9-04-2010
9-05-2010
9-06-2010
9-07-2010
9-08-2010
9-09-2010
9-10-2010
9-11-2010
9-12-2010
9-13-2010
9-14-2010
9-15-2010
9-16-2010
9-17-2010
9-18-2010
9-19-2010
9-20-2010
9-21-2010
9-22-2010
9-23-2010
9-24-2010
9-25-2010
9-26-2010
9-27-2010
9-28-2010
9-29-2010
9-30-2010
10-01-2010
10-02-2010
10-03-2010