Team:LMU-Munich/Notebook/Pathway

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== 8-24-2010 ==
== 8-24-2010 ==
-
text
+
<font color="#009933"> making competent cells</font>
 +
with Benny's protocoll & buffers
 +
 
 +
- grow the culture to an OD <sub> 600 </sub> of ~ 0,3
 +
 
 +
-> our cells: OD <sub> 600 </sub> = 0,256
 +
 
 +
- centrifuge for 10 min at 4°C by 3000rpm
 +
 
 +
- resuspend the pellet in buffer 1
 +
 
 +
-> we used 400 µl
 +
 
 +
- centrifuge for 10 min at 4°C by 2500rpm
 +
 
 +
- resuspend the pellet in less buffer 1 than before
 +
 
 +
-> we used 200 µl
 +
 
 +
- add buffer 2 in a ratio of 1:10 = buffer 2:buffer 1
 +
 
 +
-> we added 20µl of buffer 2
 +
 
 +
- incubate on ice for 10 min
 +
 
 +
-> as we probably used too little buffer, we added another 200 µl of buffer 1 and 20µl of buffer 2
 +
 
 +
- aliquote the suspension and shockfreeze it at -80°C
 +
 
 +
-> we put 50 µl in each eppendorf
 +
 
 +
 
 +
<font color="#009933"> test-transformation</font>
 +
in order to see whether the sells will work
 +
 
 +
- done with protocoll https://2010.igem.org/Team:LMU-Munich/Notebook/Protocols/3_Transformation
 +
 
 +
-> we tested with the following DNA:
 +
 
 +
::1. K098200 (biobrick) [Amp., 5 µl]
 +
 
 +
::2. pUC [Amp., 1µl]
 +
 
 +
-> as the cells are probably in a to high concentration, we thined them down with saline to an end-concentration of 10 <sup> -2 </sup>
 +
 
== 8-25-2010 ==
== 8-25-2010 ==
text
text

Revision as of 15:25, 24 August 2010


Pathway Notebook

Contents

Week Days
Monday Tuesday Wednesday Thursday Friday Saturday Sunday
31 8-02-2010 8-03-2010 8-04-2010 8-05-2010 8-06-2010 8-07-2010 8-08-2010
32 8-09-2010 8-10-2010 8-11-2010 8-12-2010 8-13-2010 8-14-2010 8-15-2010
33 8-16-2010 8-17-2010 8-18-2010 8-19-2010 8-20-2010 8-21-2010 8-22-2010
34 8-23-2010 8-24-2010 8-25-2010 8-26-2010 8-27-2010 8-28-2010 8-29-2010
35 8-30-2010 8-31-2010 9-01-2010 9-02-2010 9-03-2010 9-04-2010 9-05-2010
36 9-06-2010 9-07-2010 9-08-2010 9-09-2010 9-10-2010 9-11-2010 9-12-2010
37 9-13-2010 9-14-2010 9-15-2010 9-16-2010 9-17-2010 9-18-2010 9-19-2010
38 9-20-2010 9-21-2010 9-22-2010 9-23-2010 9-24-2010 9-25-2010 9-26-2010
39 9-27-2010 9-28-2010 9-29-2010 9-30-2010 10-01-2010 10-02-2010 10-03-2010

8-02-2010

  • Some test text here.

8-03-2010

Some test text in bold We created following tests:

  • test1
  • test2
  • test3

8-04-2010

Example of a table

header 1 header 2 header 3
row 1, cell 1 row 1, cell 2 row 1, cell 3
row 2, cell 1 row 2, cell 2 row 2, cell 3


8-05-2010

text

8-06-2010

text

8-07-2010

text

8-08-2010

text

8-09-2010

text

8-10-2010

text

8-11-2010

text

8-12-2010

text

8-13-2010

text

8-14-2010

text

8-15-2010

text

8-16-2010

text

8-17-2010

text

8-18-2010

text

8-19-2010

text

8-20-2010

text

8-21-2010

text

8-22-2010

text

8-23-2010

text

8-24-2010

making competent cells with Benny's protocoll & buffers

- grow the culture to an OD 600 of ~ 0,3

-> our cells: OD 600 = 0,256

- centrifuge for 10 min at 4°C by 3000rpm

- resuspend the pellet in buffer 1

-> we used 400 µl

- centrifuge for 10 min at 4°C by 2500rpm

- resuspend the pellet in less buffer 1 than before

-> we used 200 µl

- add buffer 2 in a ratio of 1:10 = buffer 2:buffer 1

-> we added 20µl of buffer 2

- incubate on ice for 10 min

-> as we probably used too little buffer, we added another 200 µl of buffer 1 and 20µl of buffer 2

- aliquote the suspension and shockfreeze it at -80°C

-> we put 50 µl in each eppendorf


test-transformation in order to see whether the sells will work

- done with protocoll https://2010.igem.org/Team:LMU-Munich/Notebook/Protocols/3_Transformation

-> we tested with the following DNA:

1. K098200 (biobrick) [Amp., 5 µl]
2. pUC [Amp., 1µl]

-> as the cells are probably in a to high concentration, we thined them down with saline to an end-concentration of 10 -2

8-25-2010

text

8-26-2010

text

8-27-2010

text

8-28-2010

weekend

8-29-2010

weekend

8-30-2010

text

8-31-2010

text

9-01-2010

text

9-02-2010

text

9-05-2010

text

9-04-2010

weekend

9-05-2010

weekend

9-06-2010

text

9-07-2010

text

9-08-2010

text

9-09-2010

text

9-10-2010

text

9-11-2010

weekend

9-12-2010

weekend

9-13-2010

text

9-14-2010

text

9-15-2010

text

9-16-2010

text

9-17-2010

text

9-18-2010

weekend

9-19-2010

weekend

9-20-2010

text

9-21-2010

text

9-22-2010

text

9-23-2010

text

9-24-2010

text

9-25-2010

weekend

9-26-2010

weekend

9-27-2010

text

9-28-2010

text

9-29-2010

text

9-30-2010

text

10-01-2010

text

10-02-2010

weekend

10-03-2010

weekend

 

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