"
Team:Calgary/1 August 2010
From 2010.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI. | Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI. | ||
| + | |||
| + | |||
| + | <u>Dev</u> | ||
| + | Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells. | ||
}} | }} | ||
Revision as of 16:54, 5 August 2010
Sunday August 1, 2010
Who wants to work on weekends? We do!!
Chris
Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI.
Dev
Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells.
